Zarzhetskii Yu V, Avrushchenko M Sh, Moroz V V, Gudasheva T A, Seredenin S B
V. A. Negovsky Research Institute of General Resuscitation, Moscow, Russia.
V. V. Zakusov Research Institute of Pharmacology, Moscow, Russia.
Bull Exp Biol Med. 2015 Aug;159(4):453-5. doi: 10.1007/s10517-015-2989-3. Epub 2015 Sep 21.
Neurological status was assessed and the numbers of neurons per 1 mm in pyramidal neuronal layer length in CA1 and CA4 hippocampal fields and cerebellar Purkinje cells were evaluated in albino male rats on post-resuscitation day 14 after 12-min cardiac arrest. Intraperitoneal administration of GK-2 (1 mg/kg 30 min after resuscitation and within the next 3 days with an interval of 24 h) accelerated neurological recovery of the animals, sharply reduced the intensity of cerebellar Purkinje cell death, and prevented loss of pyramidal neurons in the hippocampus. These results show the effectiveness of systemic administration of the nerve growth factor mimetic GK-2 in improving structural and functional state of the brain in the post-resuscitation period. This opens new prospects of its use for prevention and correction of post-hypoxic encephalopathy.
对白化雄性大鼠在心脏骤停12分钟后复苏第14天评估其神经状态，并评估海马CA1和CA4区锥体细胞层每1毫米长度内的神经元数量以及小脑浦肯野细胞数量。腹腔注射GK-2（复苏后30分钟给予1毫克/千克，并在接下来3天内每隔24小时给药一次）可加速动物的神经功能恢复，显著降低小脑浦肯野细胞死亡的强度，并防止海马锥体细胞丢失。这些结果表明，神经生长因子模拟物GK-2全身给药在改善复苏后时期大脑的结构和功能状态方面是有效的。这为其用于预防和纠正缺氧后脑病开辟了新的前景。
