Research Center of Neurology, Moscow, Russia.
V. V. Zakusov Research Institute of Pharmacology, Moscow, Russia.
Bull Exp Biol Med. 2020 Feb;168(4):474-478. doi: 10.1007/s10517-020-04734-8. Epub 2020 Mar 7.
Peptide mimetic of nerve growth factor GK-2 in a dose of 1-2 mg/liter improves survival of cultured rat cerebellar granule neurons exposed to the cytotoxic effect of zinc ions, but has no protective effect against copper ion cytotoxicity. Experiments on cultured rat hippocampal slices demonstrated that GK-2 did not affect reactivity of pyramidal neurons and long-term potentiation in the hippocampal field CA1 and the probability of glutamate release from presynaptic terminals in the synapses of the CA3-CA1 fields. The results suggest that GK-2 does not affect the functional properties of synaptic transmission under normal conditions, but protects neurons from the toxic effects of zinc, which creates prerequisites for GK-12 use in the treatment of neurodegenerative diseases.
神经生长因子 GK-2 的肽模拟物在 1-2 毫克/升的剂量下可提高暴露于锌离子细胞毒性作用的培养大鼠小脑颗粒神经元的存活率，但对铜离子细胞毒性没有保护作用。在培养的大鼠海马切片上的实验表明，GK-2 不影响锥体神经元的反应性以及海马场 CA1 中的长时程增强作用，也不影响 CA3-CA1 场突触中谷氨酸从突触前末梢的释放概率。结果表明，在正常条件下，GK-2 不会影响突触传递的功能特性，但可保护神经元免受锌的毒性影响，这为 GK-12 在治疗神经退行性疾病中的应用创造了条件。
