Klein Andrew P, Sattely Elizabeth S
Department of Chemical Engineering, Stanford University, Stanford, California, USA.
Nat Chem Biol. 2015 Nov;11(11):837-9. doi: 10.1038/nchembio.1914. Epub 2015 Sep 21.
Phytoalexins are abundant in edible crucifers and have important biological activities, yet no dedicated gene for their biosynthesis is known. Here, we report two new cytochromes P450 from Brassica rapa (Chinese cabbage) that catalyze unprecedented S-heterocyclizations in cyclobrassinin and spirobrassinin biosynthesis. Our results provide genetic and biochemical insights into the biosynthesis of a prominent pair of dietary metabolites and have implications for pathway discovery across >20 recently sequenced crucifers.
植保素在可食用十字花科植物中含量丰富且具有重要的生物活性，但目前尚未发现其生物合成的特定基因。在此，我们报道了来自白菜型油菜（大白菜）的两种新的细胞色素P450，它们在环芸苔素和螺环芸苔素的生物合成中催化前所未有的S-杂环化反应。我们的研究结果为一对重要膳食代谢产物的生物合成提供了遗传学和生物化学方面的见解，并对最近测序的20多种十字花科植物的代谢途径发现具有启示意义。
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