Khatri Praveen, Wally Owen, Rajcan Istvan, Dhaubhadel Sangeeta
London Research and Development Centre, Agriculture and Agri-Food Canada, London, ON, Canada.
Department of Biology, University of Western Ontario, London, ON, Canada.
Front Plant Sci. 2022 Apr 14;13:862314. doi: 10.3389/fpls.2022.862314. eCollection 2022.
Cytochrome P450 monooxygenases (P450) participate in the catalytic conversion of biological compounds in a plethora of metabolic pathways, such as the biosynthesis of alkaloids, terpenoids, phenylpropanoids, and hormones in plants. Plants utilize these metabolites for growth and defense against biotic and abiotic stress. In this study, we identified 346 P450 (GmP450) enzymes encoded by 317 genes in soybean where 26 genes produced splice variants. The genome-wide comparison of both A-type and non-A-type GmP450s for their motifs composition, gene structure, tissue-specific expression, and their chromosomal distribution were determined. Even though conserved P450 signature motifs were found in all GmP450 families, larger variation within a specific motif was observed in the non-A-type GmP450s as compared with the A-type. Here, we report that the length of variable region between two conserved motifs is exact in the members of the same family in majority of the A-type GmP450. Analyses of the transcriptomic datasets from soybean- interaction studies, quantitative trait loci (QTL) associated with resistance, and co-expression analysis identified some GmP450s that may be, in part, play an important role in partial resistance against The findings of our CYPome study provides novel insights into the functions of GmP450s and their involvements in metabolic pathways in soybean. Further experiments will elucidate their roles in general and legume-specific function.
细胞色素P450单加氧酶（P450）参与多种代谢途径中生物化合物的催化转化，例如植物中生物碱、萜类化合物、苯丙烷类化合物和激素的生物合成。植物利用这些代谢产物进行生长，并抵御生物和非生物胁迫。在本研究中，我们在大豆中鉴定出由317个基因编码的346种P450（GmP450）酶，其中26个基因产生了剪接变体。我们确定了A型和非A型GmP450在基序组成、基因结构、组织特异性表达及其染色体分布方面的全基因组比较。尽管在所有GmP450家族中都发现了保守的P450特征基序，但与A型相比，在非A型GmP450中观察到特定基序内的变异更大。在此，我们报告在大多数A型GmP450的同一家族成员中，两个保守基序之间可变区域的长度是精确的。对来自大豆相互作用研究的转录组数据集、与抗性相关的数量性状位点（QTL）以及共表达分析的分析，鉴定出一些GmP450，它们可能在对[未提及具体对象]的部分抗性中发挥重要作用。我们的CYPome研究结果为GmP450的功能及其在大豆代谢途径中的参与提供了新的见解。进一步的实验将阐明它们在一般功能和豆科植物特异性功能中的作用。
