唾液硫氰酸盐、尿酸和pH值作为吸烟者和非吸烟者牙周疾病生物标志物的体外研究

Salivary Thiocyanate, Uric Acid and pH as Biomarkers of Periodontal Disease in Tobacco Users and Non-Users- An In-Vitro Study.

作者信息

Pullishery Fawaz, Panchmal Ganesh Shenoy, Siddique Sabin

机构信息

Senior Lecturer, Department of Public Health Dentistry, Educare Institute of Dental Sciences , Chattiparamb, Kottakkal Road, Kerala, India .

Senior Professor and HOD, Department of Public Health Dentistry, Yenepoya Dental College , Yenepoya Dental College, Mangalore, India .

出版信息

J Clin Diagn Res. 2015 Jul;9(7):ZC47-50. doi: 10.7860/JCDR/2015/12783.6203. Epub 2015 Jul 1.

DOI:10.7860/JCDR/2015/12783.6203

PMID:26393205

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4551899/

Abstract

INTRODUCTION

Biomarkers of tobacco exposure are used to confirm the absorption of specific smoke constituents in a quantitative manner.

MATERIALS AND METHODS

Periodontal status was measured by Community periodontal Index (WHO). Salivary thiocyanate was determined using spectrophotometric analysis, Salivary uric acid concentration was determined using the enzymatic uric acid assay reagent. pH was measured using pH strips.

RESULTS

Proportion of participants with periodontal pockets of 4-5mm was highest among tobacco smokers (80.0%) and periodontal pockets of 6mm or more were observed to be higher among tobacco chewers (26.67%). When LOA scores among tobacco chewers were recorded 26.7% of them presented with attachment loss of 0-3mm. 46.7% of them were affected with loss of attachment of 4-5mm and loss of attachment 6-8mm was found in 16.7%. 6.7% of them were found to be having loss of attachment of 9-11mm. 3.3% of them were found to be having loss of attachment of 12mm or more. The LOA scores in non- tobacco users showed 46.7% of 0-3mm, 50.0% of 4-5mm and 3.33% of 6-8mm attachment loss. The mean level of thiocyanate level detected in saliva of tobacco smokers was 172 ± 54.7 μg/ml and 203.70± 45.7 μg/ml in tobacco chewers. The mean uric acid levels was found to be 2.54 ±0.63 (μg/dl) in smokers and 2.65 ± 0.37(μg/dl) in chewers and in non tobacco users it was 2.33 ± 0.47 μg/dl.

CONCLUSION

Tobacco users had significantly higher concentration of SCN levels than non-users.

摘要

引言

烟草暴露生物标志物用于定量确认特定烟雾成分的吸收情况。

材料与方法

采用社区牙周指数（世界卫生组织）测量牙周状况。使用分光光度法分析测定唾液硫氰酸盐。使用尿酸酶测定试剂测定唾液尿酸浓度。使用pH试纸测量pH值。

结果

在吸烟者中，牙周袋深度为4 - 5mm的参与者比例最高（80.0%），而在嚼烟者中，牙周袋深度为6mm或更深的比例更高（26.67%）。记录嚼烟者的牙周附着丧失（LOA）评分时，26.7%的人牙周附着丧失0 - 3mm，46.7%的人牙周附着丧失4 - 5mm，16.7%的人牙周附着丧失6 - 8mm，6.7%的人牙周附着丧失9 - 11mm，3.3%的人牙周附着丧失12mm或更多。非烟草使用者的LOA评分显示，46.7%的人牙周附着丧失0 - 3mm，50.0%的人牙周附着丧失4 - 5mm，3.33%的人牙周附着丧失6 - 8mm。吸烟者唾液中检测到的硫氰酸盐平均水平为172±54.7μg/ml，嚼烟者为203.70±45.7μg/ml。吸烟者的平均尿酸水平为2.54±0.63（μg/dl），嚼烟者为2.65±0.37（μg/dl），非烟草使用者为2.33±0.47μg/dl。