Josephy P David, Zahid Muhammad, Dhanoa Joban, de Souza Giovanna Brondino Duarte, Groom Hilary, Lambie Meghan
Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada.
Department of Chemistry, University of Agriculture, Faisalabad, Pakistan.
Environ Mol Mutagen. 2016 Jan;57(1):10-6. doi: 10.1002/em.21983. Epub 2015 Sep 22.
Genotoxicity data on commercial azo dyes and their components remain sparse, despite their widespread use. We have tested the mutagenicity of 2-cyano-4-nitroaniline (CNNA) and 2,6-dicyano-4-nitroaniline (CNCNNA), components of azo dyes such as Disperse Blue 165 and Disperse Red 73, in Ames test strains. Both compounds are extraordinarily potent frameshift mutagens, with much greater activity than structurally similar dihalonitroanilines and halodinitroanilines. Analysis of the responses of strains over-expressing or deficient in bioactivation enzymes shows that bacterial nitroreductase and acetyl CoA: arylamine N-acetyltransferase are important mediators of the mutagenicity of CNNA and CNCNNA.
尽管商业偶氮染料及其成分被广泛使用，但其遗传毒性数据仍然稀少。我们在艾姆斯试验菌株中测试了偶氮染料（如分散蓝165和分散红73）的成分2-氰基-4-硝基苯胺（CNNA）和2,6-二氰基-4-硝基苯胺（CNCNNA）的致突变性。这两种化合物都是极强的移码诱变剂，其活性远高于结构相似的二卤代硝基苯胺和卤代二硝基苯胺。对过表达或缺乏生物活化酶的菌株反应的分析表明，细菌硝基还原酶和乙酰辅酶A：芳胺N-乙酰转移酶是CNNA和CNCNNA致突变性的重要介质。
