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印度西南部沿海沙丘刀豆的生豆、熟豆及少孢根霉发酵豆的抗氧化活性

Antioxidant activity of raw, cooked and Rhizopus oligosporus fermented beans of Canavalia of coastal sand dunes of Southwest India.

作者信息

Niveditha Vedavyas R, Sridhar Kandikere R

机构信息

Department of Biosciences, Mangalore University, Mangalagangotri, Mangalore, 574 199 Karnataka India.

出版信息

J Food Sci Technol. 2014 Nov;51(11):3253-60. doi: 10.1007/s13197-012-0830-9. Epub 2012 Sep 4.

Abstract

The raw and processed (cooked and cooked + solid-state fermented with Rhizopus oligosporus) split beans of two landraces of coastal sand dune wild legumes (Canavalia cathartica and Canavalia maritima) of the southwest coast of India were examined for bioactive compounds (total phenolics, tannins and vitamin C) and antioxidant potential (total antioxidant activity, ferrous-ion chelating capacity, DPPH free radical-scavenging activity and reducing activity). One-way ANOVA revealed significant elevation of bioactive compounds as well as antioxidant activities in fermented beans compared to raw and cooked beans in both legumes (p < 0.001). The EC50 values in fermented beans of both legumes were significantly lowest compared to raw and cooked beans (p < 0.001). In principal component analysis, total phenolics along with antioxidant activities (total antioxidant, ferrous-ion chelating and free radical-scavenging activities) of fermented beans of C. cathartica, while total antioxidant and free radical-scavenging activities of fermented beans of C. maritima were clustered. The present study demonstrated that split beans of coastal sand dune Canavalia fermented by R. oligosporus endowed with high bioactive principles as well as antioxidant potential and thus serve as future nutraceutical source.

摘要

对印度西南海岸两种海岸沙丘野生豆科植物地方品种(刺果刀豆和滨海刀豆)的生豆以及加工处理后的豆子(煮熟的和用少孢根霉进行固态发酵后煮熟的)进行了生物活性化合物(总酚、单宁和维生素C)和抗氧化潜力(总抗氧化活性、亚铁离子螯合能力、DPPH自由基清除活性和还原活性)的检测。单因素方差分析显示,与这两种豆科植物的生豆和煮熟的豆子相比,发酵豆中的生物活性化合物以及抗氧化活性均显著提高(p < 0.001)。与生豆和煮熟的豆子相比,这两种豆科植物发酵豆的半数有效浓度(EC50)值均显著最低(p < 0.001)。在主成分分析中,刺果刀豆发酵豆的总酚以及抗氧化活性(总抗氧化、亚铁离子螯合和自由基清除活性)聚类在一起,而滨海刀豆发酵豆的总抗氧化和自由基清除活性聚类在一起。本研究表明,用少孢根霉发酵的海岸沙丘刀豆的裂豆具有高生物活性成分以及抗氧化潜力,因此可作为未来的营养保健品来源。

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