Mehdizadeh Amir, Rahimipour Ali, Farzadi Laya, Darabi Masoud, Shahnazi Vahideh, Vatankhah Amir-Mansour, Golmohamadi Zahra, Nouri Mohammad
Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran.
Iran J Reprod Med. 2011 Summer;9(3):193-8.
Follicular fluid (FF) plays an important role in oocytes and embryo development, which may contribute to IVF/ICSI success rate.
The aim of this study was to investigate the correlation between cholesteryl ester transfer protein (CETP) level in FF and the success rate of IVF/ICSI.
In a cross-sectional study, FF samples, FF samples were obtained from 100 patients referred to Tabriz Alzahra Hospital. Seventy-nine subjects underwent IVF and the remaining 21 underwent ICSI. The levels of high-density lipoprotein cholesterol (HDL-C), apolipoprotein A-I and CETP were measured using enzymatic, turbidometric and ELISA methods respectively.
Analysis of the subgroups with different levels of CETP showed a significant lower level of CETP in the subgroup with the lowest number of mature oocytes (p<0.05). The level of CETP was also considerably lower (18%, p=0.05) in subjects with<50% oocytes fertilization ratio than subjects with >70% of this ratio.
While no association was found for pregnancy, the amount of CETP in FF was associated positively to the maturity and the percentage of oocyte fertilization.
卵泡液（FF）在卵母细胞和胚胎发育中起重要作用，这可能有助于提高体外受精/卵胞浆内单精子注射（IVF/ICSI）的成功率。
本研究旨在探讨卵泡液中胆固醇酯转运蛋白（CETP）水平与IVF/ICSI成功率之间的相关性。
在一项横断面研究中，从转诊至大不里士阿尔扎赫拉医院的100例患者中获取卵泡液样本。79例受试者接受了IVF，其余21例接受了ICSI。分别采用酶法、比浊法和酶联免疫吸附测定法（ELISA）测量高密度脂蛋白胆固醇（HDL-C）、载脂蛋白A-I和CETP的水平。
对不同CETP水平亚组的分析显示，成熟卵母细胞数量最少的亚组中CETP水平显著较低（p<0.05）。卵母细胞受精率<50%的受试者中CETP水平也明显较低（18%，p=0.05），低于受精率>70%的受试者。
虽然未发现与妊娠有关联，但卵泡液中CETP的含量与卵母细胞的成熟度和受精率呈正相关。
