Shaaker Maghsod, Rahimipour Ali, Nouri Mohammad, Khanaki Korosh, Darabi Masoud, Farzadi Laya, Shahnazi Vahideh, Mehdizadeh Amir
Laboratory of Chromatography, Dept. of Biochemistry and Clinical Laboratories, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Faculty of Para Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Iran Biomed J. 2012;16(3):162-8. doi: 10.6091/ibj.1081.2012.
Fatty acids are known to be critically important in multiple biological functions. Phospholipid fatty acids of follicular fluid, an important microenvironment for the development of oocytes, may contribute to the women's fertility and the efficacy of assisted reproduction techniques. The aim of this study was to investigate the effect of fatty acid composition of follicular fluid phospholipids on women undergoing assisted reproductive techniques.
Follicular fluid samples were obtained from 100 patients, referred to Tabriz Alzahra Hospital. Seventy-nine subjects underwent in vitro fertilization (IVF) and the remaining 21 underwent intracytoplasmic sperm injection (ICSI). Total lipid of follicular fluid was extracted and fatty acids were analyzed by gas-liquid chromatography.
Saturated fatty acids (SFA, P = 0.002) and the ratio of SFA to polyunsaturated fatty acids (P = 0.001) were correlated negatively with a number of mature oocytes after age adjustment. Linoleic acid (P = 0.006) was positively correlated, while the level of arachidonic acid was negatively correlated with fertility percentage after adjustment for body mass index, sperm count, sperm motility.
Since phospholipids are one of the major components of lipid metabolism, the results of this study highlight the importance of this component in follicular fluid lipid metabolism. Consequently, it is proposed as an index in determination of the rate of success in assisted reproductive techniques such as IVF/ICSI.
脂肪酸在多种生物学功能中起着至关重要的作用。卵泡液中的磷脂脂肪酸是卵母细胞发育的重要微环境，可能对女性生育能力及辅助生殖技术的疗效有影响。本研究旨在探讨卵泡液磷脂脂肪酸组成对接受辅助生殖技术的女性的影响。
从大不里士阿尔扎赫拉医院转诊的100例患者中获取卵泡液样本。79名受试者接受体外受精（IVF），其余21名接受卵胞浆内单精子注射（ICSI）。提取卵泡液中的总脂质，并通过气液色谱法分析脂肪酸。
调整年龄后，饱和脂肪酸（SFA，P = 0.002）以及SFA与多不饱和脂肪酸的比值（P = 0.001）与成熟卵母细胞数量呈负相关。调整体重指数、精子计数、精子活力后，亚油酸（P = 0.006）呈正相关，而花生四烯酸水平与受孕率呈负相关。
由于磷脂是脂质代谢的主要成分之一，本研究结果凸显了该成分在卵泡液脂质代谢中的重要性。因此，建议将其作为确定IVF/ICSI等辅助生殖技术成功率的一个指标。
