Department of Obstetrics and Gynecology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts, USA.
Fertil Steril. 2010 May 1;93(7):2354-61. doi: 10.1016/j.fertnstert.2008.12.129. Epub 2009 Feb 20.
To investigate involvement of specific apolipoproteins in the process of human oocyte maturation and age-related infertility as molecular constituents of follicular fluid.
Laboratory-based observational study.
Basic science laboratory at a large academic institution.
PATIENT(S): Follicular fluid obtained from healthy women aged 18 to 45 years undergoing in vitro fertilization for unexplained infertility, ovulatory dysfunction, tubal disease, male factor infertility, or oocyte donation.
INTERVENTION(S): None.
MAIN OUTCOME MEASURE(S): Specific concentration of apolipoproteins and content of lipoprotein particles in follicular fluid and blood plasma as related to reproductive aging.
RESULT(S): We registered a decline of follicular apolipoprotein A1 (Apo A1) and apolipoprotein CII (Apo CII) and an increase of the apolipoprotein E (Apo E) with age, which parallels a lower number of retrieved mature oocytes in older women. Follicular apolipoprotein A1, apolipoprotein B (Apo B), apolipoprotein E, and apolipoprotein C II are present in diverse heterogeneous complexes including very-low-density lipoproteins (VLDL), intermediate-low-density lipoproteins (IDL), low-density lipoproteins (LDL), and high-density lipoproteins (HDL) that vary with patient age and differ from the blood plasma lipoprotein complexes.
CONCLUSION(S): Age-related variation in follicular apolipoprotein content and distribution in the cholesterol particles may be associated with the decrease in production of mature oocytes and the age-related decline in fertility potential.
研究特定载脂蛋白在人类卵母细胞成熟过程中的作用以及作为卵泡液分子成分的与年龄相关的不育，研究载脂蛋白在人类卵母细胞成熟过程中的作用以及作为卵泡液分子成分的与年龄相关的不育。
基于实验室的观察性研究。
大型学术机构的基础科学实验室。
18 至 45 岁的健康女性，她们因不明原因的不孕、排卵功能障碍、输卵管疾病、男性因素不孕或卵子捐赠而接受体外受精。
无。
与生殖衰老相关的卵泡液和血浆中载脂蛋白的特异性浓度和脂蛋白颗粒含量。
我们发现，随着年龄的增长，卵泡载脂蛋白 A1（Apo A1）和载脂蛋白 CII（Apo CII）的含量下降，载脂蛋白 E（Apo E）的含量增加，这与老年女性取回的成熟卵母细胞数量减少有关。卵泡载脂蛋白 A1、载脂蛋白 B（Apo B）、载脂蛋白 E 和载脂蛋白 C II 存在于不同的异质复合物中，包括极低密度脂蛋白（VLDL）、中间低密度脂蛋白（IDL）、低密度脂蛋白（LDL）和高密度脂蛋白（HDL），这些复合物随患者年龄的变化而变化，与血浆脂蛋白复合物不同。
卵泡载脂蛋白含量和胆固醇颗粒中分布的年龄相关性变化可能与成熟卵母细胞产量的减少以及与年龄相关的生育能力下降有关。
