Division of Plastic Surgery, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, P.R. China.
Division of Surgery, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, P.R. China.
Int J Oncol. 2015 Nov;47(5):1829-38. doi: 10.3892/ijo.2015.3177. Epub 2015 Sep 22.
Keloid is a type of pathological skin scar. Pathogenesis of keloid is complex and is not fully understood. lncRNA can regulate gene expression on different levels. It also participates in cell cycle regulation and cell proliferation. The present study investigated the potential biological function of lncRNA in keloid. We identified differential expression of lncRNAs and mRNAs between 3 pairs of keloid and normal skin tissue by microarray. Differentially expressed lncRNAs were validated by quantitative reverse transcriptase-PCR (qRT-PCR). Gene ontology (GO) and pathway analysis presented the characteristics of associated protein-coding genes. Additionally, a co-expression network of lncRNA and mRNA was constructed to find potential underlying regulation targets. There were 1,731 lncRNAs constantly upregulated and 782 downregulated, 1,079 mRNAs upregulated and 3,282 downregulated in keloid respectively (fold change ≥ 2.0, p<0.05). We chose, respectively, 3 upregulated and 1 downregulated lncRNA for qRT-PCR and results were consistent with microarray. Moreover, 11 pathways were related with upregulated transcripts and 44 with downregulated in keloid. The co-expression network revealed that one lncRNA was connected with numerous mRNAs, and vice versa. Furthermore, bioinformation analysis suggested that lncRNA CACNA1G-AS1 may be crucial to keloid formation. In conclusion, groups of lncRNAs were aberrantly expressed in keloid compared with normal skin tissue, which indicated that differentially expressed lncRNAs may play a key role in keloid formation. The present study provides new insights into keloid pathology and potential targets for treatment of keloid.
瘢痕疙瘩是一种病理性皮肤瘢痕。瘢痕疙瘩的发病机制复杂，尚未完全阐明。lncRNA 可以在不同水平上调节基因表达。它还参与细胞周期调控和细胞增殖。本研究旨在探讨 lncRNA 在瘢痕疙瘩中的潜在生物学功能。我们通过微阵列鉴定了 3 对瘢痕疙瘩和正常皮肤组织之间 lncRNA 和 mRNA 的差异表达。通过定量逆转录 PCR（qRT-PCR）验证差异表达的 lncRNA。基因本体论（GO）和通路分析呈现了相关蛋白编码基因的特征。此外，构建了 lncRNA 和 mRNA 的共表达网络，以寻找潜在的潜在调节靶点。瘢痕疙瘩中分别有 1731 个 lncRNA 持续上调和 782 个下调，1079 个 mRNA 上调和 3282 个下调（倍数变化≥2.0，p<0.05）。我们分别选择 3 个上调和 1 个下调的 lncRNA 进行 qRT-PCR，结果与微阵列一致。此外，有 11 条通路与上调转录物相关，44 条与下调转录物相关。共表达网络表明，一个 lncRNA 与许多 mRNAs 相连，反之亦然。此外，生物信息学分析表明，lncRNA CACNA1G-AS1 可能对瘢痕疙瘩的形成至关重要。总之，与正常皮肤组织相比，瘢痕疙瘩中存在大量 lncRNA 的异常表达，这表明差异表达的 lncRNA 可能在瘢痕疙瘩形成中发挥关键作用。本研究为瘢痕疙瘩的病理机制提供了新的见解，并为瘢痕疙瘩的治疗提供了潜在的靶点。
