Liang Zeng-Enni, Yi You-Jin, Guo Yu-Tong, Wang Ren-Cai, Hu Qiu-Long, Xiong Xing-Yao
Hunan Agricultural Product Processing Institute, Changsha, Hunan 410128, P.R. China.
College of Food Science and Technology, Hunan Agricultural University, Changsha, Hunan 410128, P.R. China.
Mol Med Rep. 2015 Nov;12(5):7629-36. doi: 10.3892/mmr.2015.4345. Epub 2015 Sep 22.
Ganoderma lucidum polysaccharides (GLPs), which were purified from the medicinal herb G. lucidum followed by ethanol precipitation, protein depletion using the Sevage assay, purification using DEAE‑cellulose (DE-52), dialysis and the use of ultrafiltration membranes, are used as an ingredient in traditional anticancer treatments in China. The aim of the current study was to evaluate the anticancer effects and investigate the underlying molecular mechanisms of GLPs on LoVo human colon cancer cells. The results demonstrated that the GLP‑mediated anticancer effect in LoVo cells was characterized by cytotoxicity, migration inhibition, enhanced DNA fragmentation, morphological alterations and increased lactate dehydrogenase release. Furthermore, the activation of caspases‑3, ‑8 and ‑9 was involved in GLP‑stimulated apoptosis. Additionally, treatment with GLPs promoted the expression of Fas and caspase‑3 proteins, whilst reducing the expression of cleaved poly(ADP‑ribose) polymerase. These data indicate that GLPs demonstrate potential antitumor activity in human colon cancer cells, predominantly through the inhibition of migration and induction of apoptosis. Furthermore, activation of the Fas/caspase-dependent apoptosis pathway is involved in the cytotoxicity of GLPs.
灵芝多糖（GLPs）是从药用植物灵芝中提取的，经过乙醇沉淀、用Sevage法去除蛋白质、用DEAE-纤维素（DE-52）纯化、透析以及使用超滤膜等步骤获得，在中国传统抗癌治疗中用作一种成分。本研究的目的是评估灵芝多糖对人LoVo结肠癌细胞的抗癌作用，并探究其潜在的分子机制。结果表明，灵芝多糖在LoVo细胞中介导的抗癌作用表现为细胞毒性、迁移抑制、DNA片段化增强、形态改变以及乳酸脱氢酶释放增加。此外，半胱天冬酶-3、-8和-9的激活参与了灵芝多糖刺激的细胞凋亡。另外，灵芝多糖处理促进了Fas和半胱天冬酶-3蛋白的表达，同时降低了裂解的聚（ADP-核糖）聚合酶的表达。这些数据表明，灵芝多糖在人结肠癌细胞中显示出潜在的抗肿瘤活性，主要是通过抑制迁移和诱导细胞凋亡。此外，Fas/半胱天冬酶依赖性凋亡途径的激活参与了灵芝多糖的细胞毒性作用。
