Namli Halide, Erdogan Özgür, Gönlüşen Gülfiliz, Kahraman Onur Evren, Aydin Halil Murat, Karabag Sevil, Tatli Ufuk
*Resident, Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Cukurova University, Adana, Turkey. †Associate Professor, Department of Oral and Maxillofacial Surgery, Faculty of Dental Medicine, Rangsit University, Pathumthani, Thailand; Formerly, Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Cukurova University, Adana, Turkey. ‡Professor, Department of Pathology, Faculty of Medicine, Cukurova University, Adana, Turkey. §Private Practice, Department of Dentistry, Acibadem Hospital Group, Adana, Turkey. ¶Associate Professor, Environmental Engineering Department and Bioengineering Division, Centre for Bioengineering, Hacettepe University, Ankara, Turkey. ‖Resident, Department of Pathology, Faculty of Medicine, Cukurova University, Adana, Turkey. #Assistant Professor, Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Cukurova University, Adana, Turkey.
Implant Dent. 2016 Feb;25(1):54-62. doi: 10.1097/ID.0000000000000334.
To evaluate the bone regeneration capacity of bone marrow-derived stem cells (BMSCs) in vertical guided augmentation of bone tissue.
The calvaria of 20 rabbits were vertically augmented with autogenous bone graft (ABG); collagen/beta-tricalcium phosphate (β-TCP) linked scaffold transplanted with 15 × 10 BMSCs; or scaffold alone (control). The augmentation materials were covered with stainless steel domes. BMSCs were isolated with Ficoll-Paque technique and applied directly without in vitro expansion. The newly formed bone was evaluated using radiodensitometric, histomorphometric, histological, and micro-computed tomographic (micro-CT) analyses after a 12-week healing period. The data excluding micro-CT assessments were compared statistically.
Radiodensitometric and bone volume parameters demonstrated increased bone formation in both BMSC group and ABG group compared with control group (P < 0.01), but difference between the BMSC and ABG groups was not significant (P > 0.05). The mean histological scores for the BMSC, ABG, and control groups were 7.44 ± 1.03, 8.44 ± 0.81, and 6.00 ± 1.10, respectively, indicating significant difference among the groups (P < 0.05).
BMSCs delivered with a collagen/β-TCP linked scaffold can provide improved new bone formation that is comparable with autogenous bone block graft through vertical guided bone regeneration technique.
评估骨髓来源干细胞（BMSCs）在垂直引导骨组织增量术中的骨再生能力。
对20只兔的颅骨进行垂直骨增量，分别采用自体骨移植（ABG）；移植15×10个BMSCs的胶原/β-磷酸三钙（β-TCP）连接支架；或仅使用支架（对照组）。增量材料用不锈钢穹顶覆盖。采用Ficoll-Paque技术分离BMSCs，直接应用，不进行体外扩增。在12周愈合期后，使用放射密度测定、组织形态计量学、组织学和显微计算机断层扫描（micro-CT）分析评估新形成的骨。对排除micro-CT评估的数据进行统计学比较。
放射密度测定和骨体积参数显示，与对照组相比，BMSC组和ABG组的骨形成均增加（P<0.01），但BMSC组和ABG组之间的差异不显著（P>0.05）。BMSC组、ABG组和对照组的平均组织学评分分别为7.44±1.03、8.44±0.81和6.00±1.10，表明各组之间存在显著差异（P<0.05）。
通过垂直引导骨再生技术，与胶原/β-TCP连接支架一起递送的BMSCs可提供与自体骨块移植相当的改善的新骨形成。
