Institute for Medicinal Plant Research "Dr. Josif Pančić", Tadeuša Košćuška 1, 11000 Belgrade, Serbia.
Department of Plant Physiology, Institute for Biological Research "Siniša Stanković", University of Belgrade, Bulevar Despota Stefana 142, 11000 Belgrade, Serbia.
Food Funct. 2015 Dec;6(12):3687-92. doi: 10.1039/c5fo00820d.
Health beneficial properties of Rosa canina species are mainly attributed to rose hips, while the leaves are usually discarded as waste. In the present study we investigated chemical constituents as well as antimicrobial and antibiofilm potential of R. canina methanolic leaf extract. Chemical analysis showed that dominant phenolic compounds are quercetin and isorhamnetin derivatives (isoquercetin and isorhamnetin-3-O-rutinoside). Among the tested bacteria, Pseudomonas aeruginosa and Salmonella typhimurium were the most susceptible to the activity of R. canina leaf extract with MIC and MBC values both 0.009 mg mL(-1). For most of the bacterial strains investigated the extract showed significantly higher activity compared to the used standard compounds streptomycin and ampicillin. Also the tested extract powerfully inhibited in vitro biofilm growth in Pseudomonas aeruginosa, Staphylococcus aureus, Leishmania monocytogenes and Escherichia coli at sub MIC-levels. With concentrations equal to 1/4 and 1/8 of MIC values biofilm growth of P. aeruginosa and E. coli was inhibited by 90%. The obtained results are significant for a wider and efficient use of R. canina leaves.
狗蔷薇属植物的有益健康特性主要归因于玫瑰果，而其叶子通常被当作废物丢弃。在本研究中，我们研究了狗蔷薇属植物甲醇叶提取物的化学成分以及抗微生物和抗生物膜特性。化学分析表明，占主导地位的酚类化合物为槲皮素和异鼠李素衍生物（异槲皮苷和异鼠李素-3-O-芸香糖苷）。在测试的细菌中，铜绿假单胞菌和鼠伤寒沙门氏菌对狗蔷薇属植物叶提取物的活性最敏感，MIC 和 MBC 值均为 0.009 mg/mL。对于大多数测试的细菌菌株，提取物的活性明显高于使用的标准化合物链霉素和氨苄西林。此外，在亚 MIC 水平下，该提取物还能强力抑制铜绿假单胞菌、金黄色葡萄球菌、单核细胞增生李斯特菌和大肠杆菌的体外生物膜生长。当浓度等于 MIC 值的 1/4 和 1/8 时，铜绿假单胞菌和大肠杆菌的生物膜生长被抑制了 90%。这些结果对于更广泛和有效地利用狗蔷薇属植物的叶子具有重要意义。
