塞尔维亚市售L. 干果实的化学特征、抗氧化及抗菌活性
Chemical Profile and Antioxidant and Antimicrobial Activity of L. Dried Fruit Commercially Available in Serbia.
作者信息
Miljković Vojkan M, Nikolić Ljubiša, Mrmošanin Jelena, Gajić Ivana, Mihajilov-Krstev Tatjana, Zvezdanović Jelena, Miljković Milena
机构信息
Faculty of Technology, University of Niš, Bulevar oslobodjenja 124, 16000 Leskovac, Serbia.
Department of Chemistry, Faculty of Sciences and Mathematics, University of Niš, Višegradska 33, 18000 Niš, Serbia.
出版信息
Int J Mol Sci. 2024 Feb 21;25(5):2518. doi: 10.3390/ijms25052518.
The aim of this work was to give as much information as possible on dried fruit that is commercially available in Serbia. In order to provide the chemical composition, the UHPLC-DAD-ESI-MS method was employed for both polar and non-polar extracts of samples obtained with a solvent mixture consisting of hexane, acetone, and ethanol in a volume ratio of 2:1:1, respectively, and 0.05% (/) butylated hydroxytoluene. In addition, the total content levels of lycopene, -carotene, total polyphenols, and flavonoids were determined by means of UV-vis spectrophotometry. The antioxidant activity was tested by applying four different methods: ABTS, DPPH, FRAP, and CUPRAC. Overall, nine compounds were identified. The results of chemical composition analysis were used as the basis for the interpretation of the calculated results for the antioxidant and antimicrobial activity. The obtained results for dried fruit extract are as follows: -carotene-7.25 [mg/100 g fruit weight]; lycopene-2.34 (mg/100 g FW); total polyphenol content (TPC)-2980 [mg GAE/kg FW]; total flavonoid content (TFC)-1454 [mg CE/kg FW]; antioxidant activity-ABTS 12.3 [μmol/100 g FW], DPPH 6.84, FRAP 52.04, and CUPRAC 15,425; and antimicrobial activity- MIC/MMC 4/0 [mg∙mL], 4/0, 4/0, 4/0, 4/4, 4/0, 2/0, and 2/0.
这项工作的目的是提供尽可能多关于塞尔维亚市场上可买到的干果的信息。为了确定其化学成分,采用超高效液相色谱-二极管阵列-电喷雾电离质谱法(UHPLC-DAD-ESI-MS)分析样品的极性和非极性提取物,样品通过分别按体积比2:1:1的己烷、丙酮和乙醇以及0.05%(/)丁基化羟基甲苯组成的混合溶剂获得。此外,通过紫外可见分光光度法测定番茄红素、β-胡萝卜素、总多酚和类黄酮的总含量水平。采用四种不同方法测试抗氧化活性:ABTS法、DPPH法、FRAP法和CUPRAC法。总体而言,共鉴定出九种化合物。化学成分分析结果用作解释抗氧化和抗菌活性计算结果的基础。干果提取物的所得结果如下:β-胡萝卜素-7.25[毫克/100克果实重量];番茄红素-2.34(毫克/100克鲜重);总多酚含量(TPC)-2980[毫克没食子酸当量/千克鲜重];总黄酮含量(TFC)-1454[毫克儿茶素当量/千克鲜重];抗氧化活性-ABTS 12.3[微摩尔/100克鲜重]、DPPH 6.84、FRAP 52.04和CUPRAC 15425;以及抗菌活性-MIC/MMC 4/0[毫克∙毫升]、4/0、4/0、4/0、4/4、4/0、2/0和2/0。