Zheng Jing, Ma Dandan, Shi Muling, Bai Junhui, Li Yinhui, Yang Jinfeng, Yang Ronghua
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, China.
School of Chemistry and Biological Engineering, Changsha University of Science and Technology, Changsha, 410004, China.
Chem Commun (Camb). 2015 Nov 21;51(90):16271-4. doi: 10.1039/c5cc06549f.
In this communication, we proposed a new enzyme-free quadratic SERS signal amplification approach for the ultrasensitive detection of circulating miRNA in human serum. Combined with miRNA-triggered hybridization chain reaction and Ag(+)-mediated cascade amplification, a limit of miRNA detection as low as 0.3 fM could be achieved. More importantly, our method is suitable for the direct detection of circulating miRNAs in human serum collected from patients with different stages of chronic lymphocytic leukemia (CLL).
在本通讯中，我们提出了一种用于超灵敏检测人血清中循环miRNA的新型无酶二次表面增强拉曼散射（SERS）信号放大方法。结合miRNA触发的杂交链式反应和银离子介导的级联放大，可实现低至0.3 fM的miRNA检测限。更重要的是，我们的方法适用于直接检测从不同慢性淋巴细胞白血病（CLL）阶段患者采集的人血清中的循环miRNA。
