Li Liansheng, Xiao Rui, Wang Qi, Rong Zhen, Zhang Xueqing, Zhou Pingkun, Fu Hanjiang, Wang Shengqi, Wang Zhidong
Anhui Medical University Anhui 230032 P R China
Department of Radiation Toxicology and Oncology, Beijing Key Laboratory for Radiobiology, Beijing Institute of Radiation Medicine Beijing 100850 P R China.
RSC Adv. 2018 Jan 29;8(10):5119-5126. doi: 10.1039/c7ra12238a.
In a large-scale radiological catastrophe, it is expected that hundreds and thousands of people could be exposed to radiation. A rapid method is required for triage of casualties to determine proper medical treatment. In this article, mice were exposed to different radiation doses and sera of mice were investigated by surface-enhanced Raman spectroscopy (SERS) and orthogonal projections to latent structure discriminant analysis (OPLS-DA) after total body irradiation (TBI). The results of the present study indicated that differences have widened over time. The different radiation groups showed a slight overlap at 24 h and 72 h but were completely distinct at the 10th day after TBI. The SERS spectrum between the normal group and the irradiated group showed a significant difference at 24 hours. The same trend was depicted in scatting score plots. Significant differences in Raman peaks were found, such as 744 and 1495 cm corresponding to riboflavin and 593 and 1204 cm corresponding to l-tryptophan. The lack of riboflavin and l-tryptophan will influence metabolism levels. Above all, these results bear potential in the development of label-free and rapid tools for on-site detection and screening of irradiation injuries.
在大规模放射灾难中，预计会有成千上万人受到辐射。需要一种快速方法对伤亡人员进行分类，以确定适当的医疗救治。在本文中，小鼠接受不同辐射剂量，全身照射（TBI）后，通过表面增强拉曼光谱（SERS）和正交投影到潜在结构判别分析（OPLS-DA）对小鼠血清进行研究。本研究结果表明，随着时间推移差异逐渐增大。不同辐射组在24小时和72小时时有轻微重叠，但在TBI后第10天完全分开。正常组和辐照组的SERS光谱在24小时时显示出显著差异。散射得分图也呈现出相同趋势。发现拉曼峰存在显著差异，例如对应核黄素（riboflavin）的744和1495 cm，以及对应L-色氨酸（l-tryptophan）的593和1204 cm。核黄素和L-色氨酸的缺乏会影响代谢水平。最重要的是，这些结果在开发用于现场检测和筛查辐射损伤的无标记快速工具方面具有潜力。
