Jain Dilpesh, Katti Neha
Department of Pharmacology, Sinhgad College of Pharmacy, Vadgaon, Pune, Maharashtra, India.
J Intercult Ethnopharmacol. 2015 Apr-Jun;4(2):143-6. doi: 10.5455/jice.20150314061404. Epub 2015 Mar 16.
Lycopene, a carotenoid and hesperidin, a flavonoid are naturally occurring in vegetables and fruits. Synergistic effect of a combination of carotenoid and flavonoid has been reported due to its antioxidant activity. Therefore, the present study was aimed to evaluate the protective effect of this combination on pylorus ligation induced ulcers in rats.
Thirty Wistar albino rats were divided into five groups (n = 6). Rats were fasted for 24 h before pylorus ligation. After 24 h of fasting the rats were treated with hesperidin (100 mg/kg) and lycopene (2 mg/kg) and their combination 1h prior to surgery. After an hour under ether anesthesia pylorus ligation was performed, after 5 h the animals were sacrificed, stomach was dissected, and gastric contents were collected and measured. Total acidity and pH of gastric content was estimated. Ulcer index was calculated, and macroscopic examination of the stomach was carried out.
The sham operated rats showed a significant increase in pH, volume of gastric content and total acidity and ulcer index. The rats pretreated with lycopene and hesperidin showed significant improvement in the ulcer conditions. However, rats treated with a combination of lycopene and hesperidin showed more significant restoration of gastric function as compared to sham operated rats. Moreover, a significant difference was also noted in rats treated with a combination as compared to lycopene and hesperidin treatment alone.
Thus experimentally the combination was seen to treat ulcers by anti-secretory, neutralizing, cytoprotective and mainly due to its antioxidant property.
番茄红素（一种类胡萝卜素）和橙皮苷（一种黄酮类化合物）天然存在于蔬菜和水果中。据报道，类胡萝卜素和黄酮类化合物组合具有协同效应，因其具有抗氧化活性。因此，本研究旨在评估该组合对幽门结扎诱导的大鼠溃疡的保护作用。
将30只Wistar白化大鼠分为五组（n = 6）。在幽门结扎前，大鼠禁食24小时。禁食24小时后，在手术前1小时，给大鼠分别注射橙皮苷（100毫克/千克）、番茄红素（2毫克/千克）及其组合。在乙醚麻醉下1小时后进行幽门结扎，5小时后处死动物，解剖胃，收集并测量胃内容物。估计胃内容物的总酸度和pH值。计算溃疡指数，并对胃进行宏观检查。
假手术组大鼠的pH值、胃内容物体积、总酸度和溃疡指数显著增加。预先用番茄红素和橙皮苷处理的大鼠溃疡状况有显著改善。然而，与假手术组大鼠相比，用番茄红素和橙皮苷组合处理的大鼠胃功能恢复更为显著。此外，与单独使用番茄红素和橙皮苷处理的大鼠相比，联合处理的大鼠也有显著差异。
因此，通过实验观察到该组合通过抗分泌、中和、细胞保护作用，主要因其抗氧化特性来治疗溃疡。
