Couto Lauren Izabel Medeiros, Wuicik William Luiz, Kuhn Ivan, Capriotti Juan Rodolfo Vilela, Repka João Carlos
Department of Orthopedics and Traumatology, Hospital Angelina Caron, Campina Grande do Sul, PR, Brazil.
Rev Bras Ortop. 2015 Jul 26;50(4):455-61. doi: 10.1016/j.rboe.2015.07.004. eCollection 2015 Jul-Aug.
To evaluate the influence of oral supplementation with arginine on regeneration of injuries due to straining of the anterior tibial muscle of rats.
Twenty-four Wistar rats of weight 492.5 ± 50.45 g were used. Injuries were induced through straining the anterior tibial muscles. The rats were separated into three groups of eight rats each. In the untreated group (UTG), after induction of injuries, the rats were observed for 24 h. In the simulation group (SG) and the arginine group (AG) respectively, the rats received isotonic saline solution and arginine solution via direct gavage, over a seven-day period. At the end of the period, blood samples were collected for serum evaluations of creatine kinase (CK), lactic dehydrogenase (LDH), aspartate aminotransferase (AST) and C-reactive protein (CRP). The right and left anterior tibial muscles were resected for histopathological evaluations on the muscle injuries, investigating edema, hemorrhage and disorganization or morphometric alteration of the muscle fibers. The tissue repair was investigated in terms of proliferation of adipose tissue, angiogenesis and collagen fibers. The ANOVA and Student's t methods were used and p ≤ 0.05 was taken to be statistically significant.
In the serum evaluations, the AG showed lower CK assay values and higher AST values. In the histopathological evaluation, the UTG presented edema and hemorrhage compatible with injuries due to strain; the SG presented edema and hemorrhage with proliferation of adipose tissue and collagen fibers; and the AG presented not only the findings of the SG but also, especially, intense angiogenesis.
Oral supplementation with arginine did not cause any significant metabolic alterations that would contraindicate its use and it induced angiogenesis during the repair of muscles injured due to strain.
评估口服补充精氨酸对大鼠胫前肌拉伤损伤再生的影响。
使用24只体重为492.5±50.45克的Wistar大鼠。通过拉伤胫前肌诱导损伤。将大鼠分为三组，每组八只。在未治疗组（UTG）中，损伤诱导后，观察大鼠24小时。在模拟组（SG）和精氨酸组（AG）中，大鼠分别通过直接灌胃接受等渗盐溶液和精氨酸溶液，为期七天。在该时间段结束时，采集血样用于血清肌酸激酶（CK）、乳酸脱氢酶（LDH）、天冬氨酸转氨酶（AST）和C反应蛋白（CRP）的评估。切除左右胫前肌进行肌肉损伤的组织病理学评估，研究水肿、出血以及肌纤维的紊乱或形态计量学改变。从脂肪组织增殖、血管生成和胶原纤维方面研究组织修复情况。使用方差分析和学生t检验方法，以p≤0.05为具有统计学意义。
在血清评估中，AG组的CK检测值较低，AST值较高。在组织病理学评估中，UTG组出现与拉伤损伤相符的水肿和出血；SG组出现水肿和出血，伴有脂肪组织和胶原纤维增殖；AG组不仅呈现出SG组的表现，而且特别出现了强烈的血管生成。
口服补充精氨酸未引起任何会妨碍其使用的显著代谢改变，并且在因拉伤而受损的肌肉修复过程中诱导了血管生成。
