使用硫化氢比率荧光探针在体内对活细胞和斑马鱼进行成像。

Imaging of living cells and zebrafish in vivo using a ratiometric fluorescent probe for hydrogen sulfide.

作者信息

Liu Tianbao, Lin Jie, Li Zhen, Lin Lin, Shen Yuning, Zhu Hailiang, Qian Yong

机构信息

State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, No.163 Xianlin Road, Nanjing 210023, China.

出版信息

Analyst. 2015 Nov 7;140(21):7165-9. doi: 10.1039/c5an00119f.

DOI:10.1039/c5an00119f

PMID:26401525

Abstract

We have developed a novel colorimetric and ratiometric fluorescence probe for the selective and sensitive monitoring of hydrogen sulfide based on a dicyanoisophorone platform. An excellent linear relationship of fluorescence intensity ratio (I637/I558) (R(2) = 0.9867) versus hydrogen sulfide concentration in the range of 1-12 μM was obtained. This probe exhibited a remarkable fluorescence response to hydrogen sulfide over other physiological thiols or biological species, which fluoresces in the red region with a large Stokes shift (172 nm). This probe was successfully utilized to monitor H2S under in vitro physiological conditions and for imaging H2S in living cells and living zebrafish in vivo.

摘要

我们基于二氰基异佛尔酮平台开发了一种新型比色和比率荧光探针，用于选择性和灵敏地监测硫化氢。在1-12 μM范围内，荧光强度比（I637/I558）（R(2) = 0.9867）与硫化氢浓度呈现出良好的线性关系。与其他生理硫醇或生物物种相比，该探针对硫化氢表现出显著的荧光响应，其在红色区域发出荧光，具有较大的斯托克斯位移（172 nm）。该探针已成功用于体外生理条件下监测H2S，并用于对活细胞和体内活斑马鱼中的H2S进行成像。