Fan Jianping, Wang Yaqin, Wang Xiaobing, Wang Pan, Tang Wei, Yuan Wenjuan, Kong Lulu, Liu Quanhong
Cell Physiol Biochem. 2015;37(3):1055-65. doi: 10.1159/000430231. Epub 2015 Sep 25.
Meconopsis horridula Hook (M. horridula) has been used as a traditional Tibetan medicine to relieve heat and pain as well as mobilize static blood, and it is recognized as a good treatment for bruises. This study is the first trial to evaluate the tumor inhibitory activity of M. horridula extract and its underlying mechanism in the hope of providing evidence to support the anticancer function of M. horridula.
M. horridula extract was cytotoxic to L1210 cells in a dose- and time-dependent manner. SEM (scanning electron microscope) observation revealed obvious morphological changes in L1210 cells after M. horridula treatment. Flow cytometry analysis demonstrated that the extract dose-dependently induced early apoptosis. Additional apoptosis parameters, such as alterations in nuclear morphology and DNA damage, were also observed. Furthermore, M. horridula treatment induced G2/M arrest. M. horridula treatment significantly increased reactive oxygen species (ROS) production, suggesting that ROS are a key factor in M. horridula-induced apoptosis. Volatile constituent detection found 15 abundant chemicals in M. horridula, which may contribute to its anticancer effect.
In conclusion, M. horridula extract induced L1210 cell apoptosis and inhibited proliferation through G2/M phase arrest, and ROS were involved in the process.
多刺绿绒蒿已被用作传统藏药，用于清热止痛、活血化瘀，被认为是治疗跌打损伤的良药。本研究是首次评估多刺绿绒蒿提取物的肿瘤抑制活性及其潜在机制的试验，旨在为支持多刺绿绒蒿的抗癌功能提供证据。
多刺绿绒蒿提取物对L1210细胞具有剂量和时间依赖性的细胞毒性。扫描电子显微镜观察显示，多刺绿绒蒿处理后L1210细胞出现明显的形态变化。流式细胞术分析表明，该提取物剂量依赖性地诱导早期凋亡。还观察到其他凋亡参数，如核形态改变和DNA损伤。此外，多刺绿绒蒿处理诱导G2/M期阻滞。多刺绿绒蒿处理显著增加活性氧（ROS）的产生，表明ROS是多刺绿绒蒿诱导凋亡的关键因素。挥发性成分检测发现多刺绿绒蒿中含有15种丰富的化学物质，这可能有助于其抗癌作用。
总之，多刺绿绒蒿提取物通过G2/M期阻滞诱导L1210细胞凋亡并抑制其增殖，且ROS参与了这一过程。
