Mai Bingjie, Gao Yiru, Li Min, Wang Xiaobing, Zhang Kun, Liu Quanhong, Xu Chuanshan, Wang Pan
Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, Ministry of Education.
National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, College of Life Sciences, Shaanxi Normal University, Xi'an.
Int J Nanomedicine. 2017 Aug 17;12:5915-5931. doi: 10.2147/IJN.S138185. eCollection 2017.
Antibiotic resistance has emerged as one of the most important determinants of outcome in patients with serious infections, along with the virulence of the underlying pathogen. Photodynamic antimicrobial chemotherapy (PACT) has been proposed as an alternative approach for the inactivation of bacteria. This study aims to evaluate the antibacterial effect of sinoporphyrin sodium (DVDMS)-mediated PACT on and multidrug resistant in vitro and in vivo.
Bacteria were incubated with DVDMS and exposed to treatment with light. After PACT treatment, colony-forming units were counted to estimate the bactericidal effect. Intracellular reactive oxygen-species production was detected by flow cytometry. Flow cytometry and fluorescence-microscopy detection of bacterial cell-membrane permeability. Enzyme-linked immunosorbent assays were used to determine expression of VEGF, TGFβ, TNFα, IL6, and bFGF factors in burn infection.
DVDMS-PACT effectively killed bacterial proliferation. Intracellular ROS levels were enhanced obviously in the PACT-treatment group. SYTO 9 and propidium iodide staining showed a decrease in the ratio of green:red fluorescence intensity in the PACT-treatment group in comparison to the control group. Enzyme-linked immunosorbent-assay results revealed that in the healing process, the expression of bFGF, TGFβ, and VEGF in the treatment group were higher than in the control group, which inhibited inflammation-factor secretion. In addition, skin-tissue bacteria were reduced after treatment.
These results indicate that DVDMS-PACT presents significant bactericidal activity and promotes wound healing after burn infections.
抗生素耐药性已成为严重感染患者预后的最重要决定因素之一，与潜在病原体的毒力相当。光动力抗菌化疗（PACT）已被提议作为一种使细菌失活的替代方法。本研究旨在评估血卟啉单甲醚（DVDMS）介导的PACT在体外和体内对[具体细菌名称]及多重耐药菌的抗菌效果。
将细菌与DVDMS孵育并进行光照处理。PACT处理后，计数菌落形成单位以评估杀菌效果。通过流式细胞术检测细胞内活性氧的产生。采用流式细胞术和荧光显微镜检测细菌细胞膜通透性。酶联免疫吸附测定法用于测定烧伤感染中血管内皮生长因子（VEGF）、转化生长因子β（TGFβ）、肿瘤坏死因子α（TNFα）、白细胞介素6（IL6）和碱性成纤维细胞生长因子（bFGF）因子的表达。
DVDMS-PACT有效抑制了细菌增殖。PACT处理组细胞内活性氧水平明显升高。SYTO 9和碘化丙啶染色显示，与对照组相比，PACT处理组绿色:红色荧光强度比值降低。酶联免疫吸附测定结果显示，在愈合过程中，治疗组bFGF、TGFβ和VEGF的表达高于对照组，且抑制了炎症因子的分泌。此外，治疗后皮肤组织中的细菌数量减少。
这些结果表明，DVDMS-PACT具有显著的杀菌活性，并能促进烧伤感染后的伤口愈合。
