术后粘连体外发育模型的创建。
The Creation of a Model for Ex Vivo Development of Postoperative Adhesions.
作者信息
Saed Ghassan M, Fletcher Nicole M, Diamond Michael P
机构信息
Department of Obstetrics and Gynecology, The C. S. Mott Center for Human Growth and Development, Wayne State University School of Medicine, Detroit, MI, USA
Department of Obstetrics and Gynecology, The C. S. Mott Center for Human Growth and Development, Wayne State University School of Medicine, Detroit, MI, USA.
出版信息
Reprod Sci. 2016 May;23(5):610-2. doi: 10.1177/1933719115607997. Epub 2015 Sep 25.
BACKGROUND
Postoperative adhesions occur in the overwhelming majority of patients after laparotomy and laparoscopy. The clinical consequences of adhesions can be devastating. Adhesions are believed to contribute to infertility and are the most common cause of small bowel obstruction and abdominal pelvic pain. Our objective is to develop a model for ex vivo development of postoperative adhesions.
METHODS
Peritoneal tissue strips were obtained from Sprague Dawley rats and were abraded by scraping the tissue surface with a scalpel. Tissues were incubated in a special medium, and visible adhesion bands were assessed. Experiments were performed in a university setting.
RESULTS
Adhesion bands were observed as early as 24 hours after abrasion. No adhesion bands were observed in the control tissue strips.
CONCLUSIONS
This is a model system for the creation of actual adhesive bands utilizing an ex vivo culture of peritoneal strips.
背景
剖腹手术和腹腔镜检查术后，绝大多数患者会发生术后粘连。粘连的临床后果可能是毁灭性的。粘连被认为会导致不孕，是小肠梗阻和腹腔盆腔疼痛的最常见原因。我们的目标是建立一个用于术后粘连体外发育的模型。
方法
从Sprague Dawley大鼠获取腹膜组织条，用手术刀刮擦组织表面进行磨损处理。将组织在特殊培养基中孵育，并评估可见的粘连带。实验在大学环境中进行。
结果
磨损后24小时即可观察到粘连带。对照组织条未观察到粘连带。
结论
这是一个利用腹膜条体外培养创建实际粘连带的模型系统。
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