Jo Yeonhwa, Choi Hoseong, Yoon Ju-Yeon, Choi Seung-Kook, Cho Won Kyong
Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea.
Virology Unit, Department of Horticultural Environment, National Institute of Horticultural and Herbal Science, RDA, Wan-Ju 565-852, Republic of Korea.
Gene. 2016 Jan 10;575(2 Pt 3):712-7. doi: 10.1016/j.gene.2015.09.051. Epub 2015 Sep 26.
Here, we identified eight Bell pepper endornavirus (BPEV) isolates from nine different pepper transcriptomes. BPEV was present with low copy numbers ranging from 0.01% to 0.18% in the host transcriptome. Phylogenetic identified two different groups of BPEV isolates. Sequence alignment of the five BPEV genomes revealed conservation of the 5' and 3' untranslated regions. Recombination analysis identified two possible recombinant events in the isolate Yolo Wonder. Single nucleotide variation profiles revealed the presence of BPEV variants within a single pepper cultivar. Taken together, this study provides phylogenetic and recombination analyses of the genus Endornavirus using pepper transcriptome data.
在此，我们从九个不同的辣椒转录组中鉴定出了八个甜椒内病毒（BPEV）分离株。BPEV在宿主转录组中的拷贝数较低，范围为0.01%至0.18%。系统发育分析确定了BPEV分离株的两个不同组。五个BPEV基因组的序列比对显示5'和3'非翻译区具有保守性。重组分析在分离株Yolo Wonder中确定了两个可能的重组事件。单核苷酸变异图谱揭示了单个辣椒品种中存在BPEV变体。综上所述，本研究利用辣椒转录组数据对内病毒属进行了系统发育和重组分析。
