Tomaszewska Ewa, Winiarska-Mieczan Anna, Dobrowolski Piotr
Department of Animal Physiology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, 20-950 Lublin, Poland.
Department of Bromatology and Food Physiology, University of Life Sciences in Lublin, 20-950, Poland.
Environ Toxicol Pharmacol. 2015 Nov;40(3):708-14. doi: 10.1016/j.etap.2015.09.002. Epub 2015 Sep 8.
Adult rats at the age of 12 weeks were divided into the control group and groups supplemented with green (GT), black (BT), red (RT), or white (WT) tea extracts. The diet (except that for the control) was mixed with 7 mg Cd/kg and 50 mg Pb/kg. The experiment lasted 12 weeks. Basal haematology and plasma biochemical parameters as well as the histomorphometrical parameters of jejunal epithelium and liver were determined. The lowest body mass was found in the RT and WT groups. Some functional (increased plasma ALT and AST, and the de Ritis coefficient) and structural changes in the liver (slight fatty degenerative changes, an increase in the intercellular space) were evident irrespective of the type of tea in the Cd and Pb poisoned rats. This toxic effect was visible especially in rats drinking black or red tea. However, the rats had no elevated LDH and ALT activities. The highest content of Cd and Pb in the liver and blood plasma was found in rats drinking red tea. Based on the results obtained, it is clear that long-term exposure of adult rats with a mature intestinal barrier to Cd and Pb contamination, under higher exposure conditions than the current estimates of weekly exposure of the general population to Cd and Pb through diet, causes a toxic effect, especially in the liver, and can change the structure of intestinal mucosa, irrespective of tea administration.
将12周龄的成年大鼠分为对照组以及补充绿茶（GT）、红茶（BT）、黑茶（RT）或白茶（WT）提取物的组。除对照组外，饲料中添加7毫克镉/千克和50毫克铅/千克。实验持续12周。测定了基础血液学和血浆生化参数以及空肠上皮和肝脏的组织形态计量学参数。RT组和WT组的体重最低。在镉和铅中毒的大鼠中，无论饮用何种茶，肝脏都出现了一些功能变化（血浆ALT和AST升高以及德瑞蒂斯系数升高）和结构变化（轻度脂肪变性变化、细胞间隙增加）。这种毒性作用在饮用红茶或黑茶的大鼠中尤为明显。然而，大鼠的LDH和ALT活性并未升高。饮用红茶的大鼠肝脏和血浆中的镉和铅含量最高。根据所得结果，很明显，成年大鼠在具有成熟肠道屏障的情况下，长期暴露于镉和铅污染环境中，且暴露条件高于目前一般人群通过饮食每周接触镉和铅的估计量，会产生毒性作用，尤其是对肝脏，并且会改变肠黏膜结构，无论是否给予茶。
