Aghaz F, Hajarian H, Shabankareh H Karami, Abdolmohammadi A
IVF Laboratory, Department of Animal Science, Faculty of Agriculture, Razi University, Kermanshah, Iran.
IVF Laboratory, Department of Animal Science, Faculty of Agriculture, Razi University, Kermanshah, Iran.
Theriogenology. 2015 Dec;84(9):1631-5. doi: 10.1016/j.theriogenology.2015.08.013. Epub 2015 Aug 31.
The purpose of this study was to evaluate the effect of sericin with different concentrations (0% [control], 0.1%, 0.5%, 1.0%, and 2.5%) added to the IVM medium on cumulus cell expansion, oocyte nuclear maturation, and subsequent embryo development in Sanjabi ewes during the breeding season. The resumption of meiosis was assessed by the frequency of germinal vesicle breakdown and the first polar body extrusion. After IVF with fresh ram semen, presumptive zygotes were cultured 8 days in potassium simplex optimization medium supplemented by amino acids, and the percentages developing to the two-cell and blastocyst stages were measured as the indicators of early embryonic developmental competence. More cumulus-oocyte complexes matured with 0.5% sericin underwent germinal vesicle breakdown and reached metaphase II stage compared with the control cumulus-oocyte complexes matured without sericin (P ≤ 0.05). The present findings indicated that supplementation with 0.5% sericin during the maturation culture may improve the nuclear maturation and the cumulus cell expansion. Furthermore, the percentage of blastocysts obtained from 0.5% and 0.1% sericin (37.8 ± 1.76% and 34.8 ± 1.09%, respectively) was higher (P ≤ 0.05) than that of the control medium (29.60 ± 1.67%). However, addition of 1% and 2.5% of sericin to the IVM medium oocytes had a negative effect on nuclear maturation and cumulus cell expansion. Furthermore, the percentage of cleavage and blastocyst rate was significantly lower in the 1% and 2.5% sericin groups than in the control group. These findings showed that supplementation of IVM medium with 0.5% sericin may improve the meiotic competence of oocytes and early embryonic development in Sanjabi ewes during the breeding season.
本研究的目的是评估在繁殖季节向桑贾比母羊的体外成熟（IVM）培养基中添加不同浓度（0%[对照]、0.1%、0.5%、1.0%和2.5%）的丝胶蛋白对卵丘细胞扩展、卵母细胞核成熟以及随后胚胎发育的影响。通过生发泡破裂频率和第一极体排出情况评估减数分裂的恢复。用新鲜公羊精液进行体外受精后，将假定的合子在添加氨基酸的钾离子简单优化培养基中培养8天，并将发育至二细胞和囊胚阶段的百分比作为早期胚胎发育能力的指标进行测定。与未添加丝胶蛋白而成熟的对照卵丘 - 卵母细胞复合体相比，添加0.5%丝胶蛋白成熟的更多卵丘 - 卵母细胞复合体发生了生发泡破裂并达到了中期II阶段（P≤0.05）。目前的研究结果表明，在成熟培养期间添加0.5%丝胶蛋白可能会改善核成熟和卵丘细胞扩展。此外，从添加0.5%和0.1%丝胶蛋白的培养基中获得的囊胚百分比（分别为37.8±1.76%和34.8±1.09%）高于对照培养基（29.60±1.67%）（P≤0.05）。然而，向IVM培养基的卵母细胞中添加1%和2.5%的丝胶蛋白对核成熟和卵丘细胞扩展有负面影响。此外，1%和2.5%丝胶蛋白组的卵裂率和囊胚率明显低于对照组。这些研究结果表明，在繁殖季节向桑贾比母羊的IVM培养基中添加0.5%丝胶蛋白可能会提高卵母细胞的减数分裂能力和早期胚胎发育。
