Rodrigues Andreia C M, Gravato Carlos, Quintaneiro Carla, Barata Carlos, Soares Amadeu M V M, Pestana João L T
Departamento de Biologia & CESAM, Universidade de Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal; Department of Environmental Chemistry (IDAEA-CSIC), Jordi Girona, 18-26, 08034 Barcelona, Spain.
Departamento de Biologia & CESAM, Universidade de Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal.
Environ Pollut. 2015 Dec;207:273-9. doi: 10.1016/j.envpol.2015.09.035. Epub 2015 Sep 25.
Integrative studies focused on sub-organismal responses to pyrethroid exposure are important to understand life history responses. In this study, the ecotoxicological effects of esfenvalerate (ESF) on Chironomus riparius were assessed using five biochemical biomarkers related to neurophysiological function (acetylcholinesterase) and oxidative stress (catalase; glutathione-S-transferase; total glutathione and lipid peroxidation). In addition, effects on cellular energy allocation were assessed and all results were compared with organismal level responses (larval growth, emergence and sex ratio). Exposure to sub-lethal concentrations of ESF caused the failure of C. riparius antioxidant defenses (inhibition of catalase activity and decreased levels of total glutathione), which was reflected as oxidative damage. C. riparius energy budget was decreased by exposure to ESF due to an increased energy consumption. Life cycle tests showed that exposure to ESF impaired C. riparius developmental rates and increased male:female ratios, thereby confirming its toxicity and potential population level effects at environmentally relevant concentrations.
专注于拟除虫菊酯暴露的亚生物体反应的综合研究对于理解生活史反应很重要。在本研究中，使用与神经生理功能（乙酰胆碱酯酶）和氧化应激（过氧化氢酶、谷胱甘肽 - S - 转移酶、总谷胱甘肽和脂质过氧化）相关的五种生化生物标志物评估了乙氰菊酯（ESF）对摇蚊的生态毒理学影响。此外，评估了对细胞能量分配的影响，并将所有结果与生物体水平的反应（幼虫生长、羽化和性别比例）进行了比较。暴露于亚致死浓度的ESF导致摇蚊抗氧化防御失效（过氧化氢酶活性受到抑制，总谷胱甘肽水平降低），这表现为氧化损伤。由于能量消耗增加，暴露于ESF使摇蚊的能量预算减少。生命周期试验表明，暴露于ESF会损害摇蚊的发育速率并增加雄雌比例，从而证实了其在环境相关浓度下的毒性和潜在的种群水平影响。
