Black L A, Higgins D P, Govendir M
McMaster Building B14, Faculty of Veterinary Science, The University of Sydney, New South Wales, Australia.
Aust Vet J. 2015 Nov;93(11):420-3. doi: 10.1111/avj.12364. Epub 2015 Sep 27.
To determine the in vitro susceptibilities of koala isolates of Chlamydia pecorum to enrofloxacin and chloramphenicol, which are frequently used to treat koalas with chlamydiosis, and florfenicol, a derivative of chloramphenicol.
The in vitro susceptibilities were determined by culturing three stored isolates and seven clinical swabs of C. pecorum. Susceptibility testing was undertaken using cycloheximide-treated buffalo green monkey kidney cells in 96 well microtitre plates.
The minimum inhibitory concentrations (MICs) for all isolates were 0.25-0.50 µg/mL (enrofloxacin), 1-2 µg/mL (chloramphenicol), and 1-2 µg/mL (florfenicol). Minimum bactericidal concentration (MBC) values for five isolates were also determined and were within one two-fold dilution of MICs. The MICs and MBCs of these antimicrobials were within ranges previously reported for other chlamydial species.
When combined with previously published pharmacokinetic data, the in vitro susceptibility results support chloramphenicol as a more appropriate treatment option than enrofloxacin for koalas with chlamydiosis. The susceptibility results also indicate florfenicol may be an appropriate treatment option for koalas with chlamydiosis, warranting further investigation.
确定考拉源嗜肺衣原体分离株对恩诺沙星、氯霉素（常用于治疗患衣原体病的考拉）以及氯霉素衍生物氟苯尼考的体外敏感性。
通过培养3株保存的嗜肺衣原体分离株和7份临床拭子来测定体外敏感性。在96孔微量滴定板中，使用环己酰亚胺处理的水牛绿猴肾细胞进行药敏试验。
所有分离株的最低抑菌浓度（MIC）为0.25 - 0.50μg/mL（恩诺沙星）、1 - 2μg/mL（氯霉素）和1 - 2μg/mL（氟苯尼考）。还测定了5株分离株的最低杀菌浓度（MBC）值，且均在MIC的1倍或2倍稀释范围内。这些抗菌药物的MIC和MBC在先前报道的其他衣原体物种的范围内。
结合先前公布的药代动力学数据，体外药敏结果支持对于患衣原体病的考拉，氯霉素比恩诺沙星是更合适的治疗选择。药敏结果还表明氟苯尼考可能是治疗患衣原体病考拉的合适选择，值得进一步研究。
