壳寡糖偶联物抑制细菌生物膜相关的氟苯尼考耐药性。

Chitosan Oligosaccharides Coupling Inhibits Bacterial Biofilm-Related Antibiotic Resistance against Florfenicol.

机构信息

College of Life Science, Sichuan Normal University, Chengdu 610101, China.

State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.

出版信息

Molecules. 2020 Dec 21;25(24):6043. doi: 10.3390/molecules25246043.

DOI:10.3390/molecules25246043

PMID:33371321

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7767115/

Abstract

The formation of bacterial biofilms has increased the resistance of bacteria to various environmental factors and is tightly associated with many persistent and chronic bacterial infections. Herein we design a strategy conjugating florfenicol, an antibiotic commonly used in the treatment of , with the antimicrobial biomaterial, chitosan oligosaccharides. The results demonstrated that the florfenicol-COS conjugate (F-COS) efficiently eradicated the mature biofilm, apparently inhibiting drug resistance to florfenicol. A quantity of 250 μg/mL F-COS showed effective inhibitory activity against planktonic cells and biofilm of the bacteria, and a 4-fold improvement of the F-COS compared to unmodified florfenicol was observed. Furthermore, the conjugate showed a broad-spectrum activity against both Gram-positive and Gram-negative bacteria. It suggested that F-COS might have a potential for application in the treatment of biofilm-related infections.

摘要

细菌生物膜的形成增加了细菌对各种环境因素的抵抗力，并与许多持续性和慢性细菌感染密切相关。在此，我们设计了一种策略，将抗生素氟苯尼考与抗菌生物材料壳寡糖偶联。结果表明，氟苯尼考-COS 偶联物（F-COS）有效地根除了成熟的生物膜，明显抑制了氟苯尼考的耐药性。250μg/mL 的 F-COS 对浮游细胞和细菌生物膜表现出有效的抑制活性，与未修饰的氟苯尼考相比，F-COS 的活性提高了 4 倍。此外，该偶联物对革兰氏阳性菌和革兰氏阴性菌均表现出广谱活性。这表明 F-COS 可能具有治疗生物膜相关感染的应用潜力。