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呼吸底物对腐败希瓦氏菌细胞色素含量的影响。

Influence of respiratory substrate on the cytochrome content of Shewanella putrefaciens.

作者信息

Morris C J, Gibson D M, Ward F B

机构信息

Department of Microbiology, University of Edinburgh, U.K.

出版信息

FEMS Microbiol Lett. 1990 Jun 1;57(3):259-62. doi: 10.1016/0378-1097(90)90077-4.

DOI:10.1016/0378-1097(90)90077-4
PMID:2210338
Abstract

Shewanella putrefaciens can use trimethylamine oxide (TMAO) as electron acceptor under anoxic conditions. The associated cytochromes induced during growth under various respiratory conditions have been separated by liquid chromatography (DEAE Sepharose CL6b) and SDS-PAGE and characterized spectrophotometrically and by redox potentiometry. Two major low potential cytochromes and at least three minor low potential cytochromes, likely to be involved in TMAO reduction, were found. No cytochrome specific for TMAO reductase was found.

摘要

腐败希瓦氏菌在缺氧条件下可利用氧化三甲胺(TMAO)作为电子受体。在各种呼吸条件下生长过程中诱导产生的相关细胞色素已通过液相色谱(DEAE琼脂糖CL6b)和SDS-PAGE分离,并通过分光光度法和氧化还原电位测定法进行了表征。发现了两种主要的低电位细胞色素和至少三种次要的低电位细胞色素,它们可能参与TMAO的还原。未发现对TMAO还原酶具有特异性的细胞色素。

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