Belduz Ali Osman, Canakci Sabriye, Chan Kok-Gan, Kahar Ummirul Mukminin, Chan Chia Sing, Yaakop Amira Suriaty, Goh Kian Mau
Faculty of Sciences, Department of Biology, Karadeniz Technical University, 61080 Trabzon, Turkey.
Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Stand Genomic Sci. 2015 Sep 26;10:70. doi: 10.1186/s40793-015-0065-2. eCollection 2015.
Species of Anoxybacillus are thermophiles and, therefore, their enzymes are suitable for many biotechnological applications. Anoxybacillus ayderensis AB04(T) (= NCIMB 13972(T) = NCCB 100050(T)) was isolated from the Ayder hot spring in Rize, Turkey, and is one of the earliest described Anoxybacillus type strains. The present work reports the cellular features of A. ayderensis AB04(T), together with a high-quality draft genome sequence and its annotation. The genome is 2,832,347 bp long (74 contigs) and contains 2,895 protein-coding sequences and 103 RNA genes including 14 rRNAs, 88 tRNAs, and 1 tmRNA. Based on the genome annotation of strain AB04(T), we identified genes encoding various glycoside hydrolases that are important for carbohydrate-related industries, which we compared with those of other, sequenced Anoxybacillus spp. Insights into under-explored industrially applicable enzymes and the possible applications of strain AB04(T) were also described.
嗜热栖热菌属的菌种是嗜热菌,因此,它们的酶适用于许多生物技术应用。阿伊德嗜热栖热菌AB04(T)(=NCIMB 13972(T)=NCCB 100050(T))是从土耳其里泽的阿伊德温泉中分离出来的,是最早描述的嗜热栖热菌属模式菌株之一。本研究报告了阿伊德嗜热栖热菌AB04(T)的细胞特征,以及高质量的基因组草图序列及其注释。该基因组长度为2,832,347 bp(74个重叠群),包含2,895个蛋白质编码序列和103个RNA基因,包括14个rRNA、88个tRNA和1个tmRNA。基于菌株AB04(T)的基因组注释,我们鉴定了编码各种糖苷水解酶的基因,这些基因对碳水化合物相关产业很重要,并将其与其他已测序的嗜热栖热菌属菌种的基因进行了比较。还描述了对未充分探索的工业适用酶的见解以及菌株AB04(T)的可能应用。