基于基因组的重新分类：将 Kamchatkensis 属的 Kevbrin 等（2005 年）更改为 Ayderensis 属的 Dulger 等（2004 年）的后期异名。

Genome-based reclassification of Anoxybacillus kamchatkensis Kevbrin et al. 2005 as a later heterotypic synonym of Anoxybacillus ayderensis Dulger et al. 2004.

机构信息

Department of Molecular Biology and Genetics, Faculty of Science, Karadeniz Technical University, 61080, Trabzon, Turkey.

Department of Biology, Faculty of Science, Karadeniz Technical University, 61080, Trabzon, Turkey.

出版信息

Arch Microbiol. 2022 Sep 10;204(10):613. doi: 10.1007/s00203-022-03201-4.

DOI:10.1007/s00203-022-03201-4

PMID:36087143

Abstract

In this study, we aimed to clarify the taxonomic positions of Anoxybacillus kamchatkensis DSM 14988 and Anoxybacillus ayderensis AB04 using whole-genome phylogenetic analysis, biochemical and chemotaxonomic characteristics. In phylogenetic trees drawn using whole-genome sequences and 16S rRNA gene sequences, A. kamchatkensis DSM 14988 and A. ayderensis AB04 clade together and showed high sequence similarity (99.6%) based on 16S rRNA gene. The average amino acid identity, average nucleotide identity and digital DNA-DNA hybridization values between A. kamchatkensis DSM 14988 and A. ayderensis AB04 were found to be greater than the threshold values for species demarcation. Most phenotypic and chemotaxonomic features between both species were almost identical except for a few exceptions. The present results show that A. kamchatkensis DSM 14988 is a later heterotypic synonym of A. ayderensis AB04.

摘要

在这项研究中，我们旨在通过全基因组系统发育分析、生化和化学分类学特征来阐明 Kamchatkensis 芽孢杆菌 DSM 14988 和 Aydar 芽孢杆菌 AB04 的分类地位。在全基因组序列和 16S rRNA 基因序列绘制的系统发育树中，Kamchatkensis 芽孢杆菌 DSM 14988 和 Aydar 芽孢杆菌 AB04 聚为一簇，并且基于 16S rRNA 基因显示出高度的序列相似性（99.6%）。Kamchatkensis 芽孢杆菌 DSM 14988 和 Aydar 芽孢杆菌 AB04 之间的平均氨基酸同一性、平均核苷酸同一性和数字 DNA-DNA 杂交值大于种间划分的阈值。除了少数例外，这两个物种之间的大多数表型和化学分类特征几乎相同。目前的结果表明，Kamchatkensis 芽孢杆菌 DSM 14988 是 Aydar 芽孢杆菌 AB04 的后异名。