Ulrich Craig, Quilici David R, Schlauch Karen A, Buxton Iain L O
Department of Pharmacology, University of Nevada School of Medicine, NV 89557, USA.
Reno Proteomics Core, University of Nevada, NV 89557, USA.
Integr Mol Med. 2015 Aug;2(4):261-269. doi: 10.15761/imm.1000152.
The molecular mechanisms involved in human uterine quiescence during gestation and the induction of labor at term or preterm are not completely known. Preterm delivery is associated with major morbidity and mortality and current efforts to prevent delivery until term are largely ineffective. Identification and semi-quantification of proteomic changes in uterine smooth muscle during pregnancy will allow for targeted research into how quiescence is maintained and what changes are associated with induction of labor. Examining preterm labor in this context will provide potential therapeutic targets for the management of preterm labor. We have recently performed two dimensional liquid chromatography coupled with tandem mass spectrometry on myometrial proteins isolated from pregnant patients in labor, pregnant patients not in labor, and pregnant patients in labor preterm. Using a conservative false discovery rate of 1% we have identified 2132 protein groups using this method and semi-quantitative spectral counting shows 201 proteins that have disparate levels of expression in preterm laboring samples. To our knowledge this is the first large scale proteomic study examining human uterine smooth muscle and this initial work has provided a target list for future experiments that can address how changing protein levels are involved in the induction of labor at term and preterm.
妊娠期间人类子宫静止以及足月或早产时分娩发动所涉及的分子机制尚未完全明确。早产与严重的发病率和死亡率相关，目前在足月前预防分娩的努力大多无效。识别和半定量妊娠期间子宫平滑肌蛋白质组的变化，将有助于有针对性地研究子宫静止是如何维持的，以及哪些变化与分娩发动有关。在此背景下研究早产将为早产管理提供潜在的治疗靶点。我们最近对从临产的孕妇、未临产的孕妇以及早产临产的孕妇分离出的子宫肌层蛋白质进行了二维液相色谱-串联质谱分析。使用1%的保守错误发现率，我们用这种方法鉴定出了2132个蛋白质组，半定量光谱计数显示有201种蛋白质在早产临产样本中的表达水平不同。据我们所知，这是第一项研究人类子宫平滑肌的大规模蛋白质组学研究，这项初步工作为未来的实验提供了一个目标清单，这些实验可以探讨蛋白质水平的变化如何参与足月和早产时的分娩发动。
