Ramis Margarita R, Sarubbo Fiorella, Terrasa Juan L, Moranta David, Aparicio Sara, Miralles Antonio, Esteban Susana
1 Laboratorio de Neurofisiología, Departamento de Biología, Universitat de les Illes Balears (UIB) , Palma de Mallorca, Spain .
2 Laboratorio de Infección e Inmunidad, Fundación de Investigación Sanitaria de las Islas Baleares (FISIB)-Instituto de Investigación Sanitaria de Palma (IdISPa), Centro de Investigación Biomédica en Red Enfermedades Respiratorias (CIBERES), Hospital Joan March , Mallorca, Spain .
Rejuvenation Res. 2016 Apr;19(2):159-71. doi: 10.1089/rej.2015.1685. Epub 2016 Feb 2.
Limiting enzymes in the synthesis of brain monoamines seems to be susceptible to oxidative damage, one of the most important factors in aging. It has been suggested that the use of anti-oxidants can reduce the rate of free radical production related with aging and the associated damage. Therefore, this study aims to analyze the effects of the chronic treatments with the anti-oxidant α-tocopherol (vitamin E) on central monoamines (high-performance liquid chromatography [HPLC] analysis) mediating cognitive functions, as well as on the evaluation of memory and motor abilities in old rats measured by radial maze, Barnes maze, novel object recognition test, and rotarod test. Results show that α-tocopherol significantly increased in a dose- and/or time-dependent manner the synthesis rate and the levels of monoaminergic neurotransmitters (serotonin, dopamine, and noradrenaline) in the hippocampus and striatum, brain regions involved in memory processing and motor coordination. These positive neurochemical effects, largely due to an increased activity of the limiting enzymes in monoamines synthesis, tryptophan hydroxylase and tyrosine hydroxylase, were accompanied by an improvement in cognitive and motor abilities in old rats. Altogether these findings suggest that α-tocopherol exhibits neuroprotective actions in old rats; thus, diets with α-tocopherol might represent a promising strategy to mitigate or delay the cognitive and motor decline associate with aging and related-diseases.
大脑单胺合成中的限速酶似乎易受氧化损伤影响，氧化损伤是衰老过程中最重要的因素之一。有人提出，使用抗氧化剂可以降低与衰老相关的自由基产生速率以及相关损伤。因此，本研究旨在分析用抗氧化剂α-生育酚（维生素E）进行长期治疗对介导认知功能的中枢单胺（高效液相色谱[HPLC]分析）的影响，以及对通过放射状迷宫、巴恩斯迷宫、新物体识别测试和转棒试验测量的老年大鼠记忆和运动能力的评估。结果表明，α-生育酚以剂量和/或时间依赖性方式显著提高了海马体和纹状体中（参与记忆处理和运动协调的脑区）单胺能神经递质（血清素、多巴胺和去甲肾上腺素）的合成速率和水平。这些积极的神经化学效应，很大程度上归因于单胺合成中的限速酶色氨酸羟化酶和酪氨酸羟化酶活性增加，同时老年大鼠的认知和运动能力也得到改善。这些发现共同表明，α-生育酚在老年大鼠中具有神经保护作用；因此，含有α-生育酚的饮食可能是减轻或延缓与衰老及相关疾病相关的认知和运动衰退的一种有前景的策略。
