Laboratorio de Neurofisiología, Departamento de Biología, Instituto Universitario de Investigación en Ciencias de la Salud, Universidad de las Islas Baleares (UIB), Ctra. Valldemossa Km 7,5, E-07122, Palma de Mallorca, Spain.
J Neuroimmune Pharmacol. 2018 Mar;13(1):24-38. doi: 10.1007/s11481-017-9759-0. Epub 2017 Aug 14.
Polyphenols have beneficial neurological effects delaying cognitive and motor decline in aging due to their antioxidant, antiinflammatory and neuroprotective properties. These effects could be related to SIRT1 activation (implicated in synaptic plasticity, memory and inflammation) and monoaminergic synthesis modulation. In this work, we studied in old male rats, the in vivo effects of long-term administration of different polyphenols (silymarin, quercetin and naringenin; 20 mg/kg/day i.p, 4 weeks) (Sprague-Dawley, 18 months) on cognition and motor coordination. We also analyzed in different brain regions: tryptophan hydroxylase (TPH) and tyrosine hydroxylase (TH) activities, which mediate central monoaminergic neurotransmitters synthesis; and immunoreactivities of SIRT1 and NF-κB (total and acetylated forms). Results indicated that chronic polyphenolic treatments showed restorative effects on cognition and motor coordination consistently with the biochemical and molecular results. Polyphenols reversed the age-induced deficits in monoaminergic neurotransmitters (serotonin, noradrenaline, and dopamine), increasing TPH and TH activity. In addition, polyphenolic treatments increased SIRT1 levels and decreased NF-κB levels in hippocampus. These results confirm polyphenolic treatments as a valuable potential therapeutic strategy for attenuating inflamm-aging and brain function decline.
多酚具有神经保护作用，能抗氧化、抗炎，可延迟衰老引起的认知和运动能力下降，其有益的神经作用与 SIRT1 激活（与突触可塑性、记忆和炎症有关）和单胺能合成调节有关。在这项工作中，我们研究了长期（4 周）给予不同多酚（水飞蓟素、槲皮素和柚皮苷；20mg/kg/天，腹腔注射）对老年雄性大鼠认知和运动协调的体内影响。我们还分析了不同脑区：色氨酸羟化酶（TPH）和酪氨酸羟化酶（TH）的活性，它们介导中枢单胺能神经递质的合成；以及 SIRT1 和 NF-κB（总形式和乙酰化形式）的免疫反应性。结果表明，慢性多酚治疗对认知和运动协调具有恢复作用，与生化和分子结果一致。多酚逆转了单胺能神经递质（5-羟色胺、去甲肾上腺素和多巴胺）的年龄诱导缺陷，增加了 TPH 和 TH 的活性。此外，多酚治疗增加了海马体中的 SIRT1 水平，降低了 NF-κB 水平。这些结果证实了多酚治疗作为一种有价值的潜在治疗策略，可减轻炎症衰老和大脑功能下降。
