Orlando Giustino, Chiavaroli Annalisa, Leone Sheila, Brunetti Luigi, Politi Matteo, Menghini Luigi, Recinella Lucia, Ferrante Claudio
Department of Pharmacy, Università degli Studi "Gabriele d'Annunzio", via dei Vestini 31, 66100 Chieti, Italy.
Antioxidants (Basel). 2019 Nov 29;8(12):602. doi: 10.3390/antiox8120602.
Parkinson's disease (PD) is the most common and progressive neurodegenerative and oxidative stress-related disorder, characterized by a dramatic loss of dopamine (DA) neurons in the nigrostriatal tissue. The first-line drug for PD treatment is represented by l-dopa, although clinical and preclinical studies pointed out the potential efficacy of medicinal plant- and food-derived antioxidants as brain protective agents. In this regard, the potential application of , , and extracts is of noteworthy interest, despite a lack of information in the scientific literature as regards their effect on striatal DA level.
The protective effects of , and water extracts were investigated on HypoE22 cells and isolated rat striatum specimens challenged with 6-hydroxydopamine (6-OH-DA). The extract effects against lactate dehydrogenase (LDH), nitrites, and 8-iso-prostaglandin(PG)F2α were evaluated using either single-extract treatments or a treatment with a pharmacological association. Additionally, the turnover of DA was measured.
The pharmacological association of the extracts was the most effective in contrasting the upregulated LDH and nitrite levels and in reducing striatal DA turnover.
The present findings corroborate the rational for the traditional use of , , and extracts, supporting their pharmacological association in order to improve their protective effects.
帕金森病（PD）是最常见的进行性神经退行性疾病，与氧化应激相关，其特征是黑质纹状体组织中多巴胺（DA）神经元大量丧失。左旋多巴是治疗PD的一线药物，尽管临床和临床前研究指出药用植物和食物来源的抗氧化剂作为脑保护剂的潜在功效。在这方面，尽管科学文献中缺乏关于它们对纹状体DA水平影响的信息，但[具体植物名称1]、[具体植物名称2]和[具体植物名称3]提取物的潜在应用仍值得关注。
研究了[具体植物名称1]、[具体植物名称2]和[具体植物名称3]水提取物对用6-羟基多巴胺（6-OH-DA）攻击的HypoE22细胞和离体大鼠纹状体标本的保护作用。使用单一提取物处理或药理联合处理评估提取物对乳酸脱氢酶（LDH）、亚硝酸盐和8-异前列腺素（PG）F2α的影响。此外，还测量了DA的周转率。
提取物的药理联合在对抗上调的LDH和亚硝酸盐水平以及降低纹状体DA周转率方面最有效。
本研究结果证实了传统使用[具体植物名称1]、[具体植物名称2]和[具体植物名称3]提取物的合理性，支持它们的药理联合以提高其保护作用。
