Todd L S, Hardy J C, Stringer M F, Bartholomew B A
Food Hygiene Laboratory, Central Public Health Laboratory, London, U.K.
Int J Food Microbiol. 1989 Nov;9(3):145-56. doi: 10.1016/0168-1605(89)90085-8.
Toxin production by four strains of Aeromonas hydrophila grown at 30 and 37 degrees C in two laboratory media and prawn purée was studied. Three different cell lines were used to test for cytotoxic activity, haemolytic activity was tested against rabbit and guinea pig erythrocytes, proteolytic activity was assayed with azo-casein and enterotoxic activity using the suckling mouse assay. Results showed reduced cytotoxic and haemolytic activities in prawn purée compared with the two media, but in most cases increased proteolytic activity. No enterotoxic activity was observed in prawn purée although it was occasionally detectable in both laboratory media.
研究了四株嗜水气单胞菌在两种实验室培养基和对虾泥中于30℃和37℃培养时的毒素产生情况。使用三种不同的细胞系测试细胞毒性活性，针对兔和豚鼠红细胞测试溶血活性，用偶氮酪蛋白测定蛋白水解活性，并使用乳鼠试验测定肠毒素活性。结果显示，与两种培养基相比，对虾泥中的细胞毒性和溶血活性降低，但在大多数情况下蛋白水解活性增加。在对虾泥中未观察到肠毒素活性，尽管在两种实验室培养基中偶尔可检测到。
