Beaudoin Cloutier Chanel, Guignard Rina, Bernard Geneviève, Gauvin Robert, Larouche Danielle, Lavoie Amélie, Lacroix Dan, Moulin Véronique J, Germain Lucie, Auger François A
1 Centre de recherche du CHU de Québec-Université Laval, axe médecine régénératrice and Centre de recherche en organogénèse expérimentale de l'Université Laval/LOEX , Quebec, Quebec, Canada .
2 Department of Surgery, Faculty of Medicine, Université Laval , Quebec, Quebec, Canada .
Tissue Eng Part C Methods. 2015 Dec;21(12):1297-305. doi: 10.1089/ten.TEC.2015.0258.
Our bilayered self-assembled skin substitutes (SASS) are skin substitutes showing a structure and functionality very similar to native human skin. These constructs are used, in life-threatening burn wounds, as permanent autologous grafts for the treatment of such affected patients even though their production is exacting. We thus intended to shorten their current production time to improve their clinical applicability. A self-assembled decellularized dermal matrix (DM) was used. It allowed the production of an autologous skin substitute from patient's cells. The characterization of SASS reconstructed using a decellularized dermal matrix (SASS-DM) was performed by histology, immunofluorescence, transmission electron microscopy, and uniaxial tensile analysis. Using the SASS-DM, it was possible to reduce the standard production time from about 8 to 4 and a half weeks. The structure, cell differentiation, and mechanical properties of the new skin substitutes were shown to be similar to the SASS. The decellularization process had no influence on the final microstructure and mechanical properties of the DM. This model, by enabling the production of a skin substitute in a shorter time frame without compromising its intrinsic tissue properties, represents a promising addition to the currently available burn and wound treatments.
我们的双层自组装皮肤替代物（SASS）是一种皮肤替代物，其结构和功能与天然人类皮肤非常相似。尽管这些构建体的生产要求很高，但在危及生命的烧伤创面中，它们被用作永久性自体移植物来治疗此类受影响的患者。因此，我们打算缩短其当前的生产时间，以提高其临床适用性。使用了一种自组装的脱细胞真皮基质（DM）。它使得能够从患者细胞中生产出自体皮肤替代物。使用脱细胞真皮基质重建的SASS（SASS-DM）通过组织学、免疫荧光、透射电子显微镜和单轴拉伸分析进行表征。使用SASS-DM，可以将标准生产时间从大约8周缩短至4周半。新皮肤替代物的结构、细胞分化和力学性能被证明与SASS相似。脱细胞过程对DM的最终微观结构和力学性能没有影响。该模型通过能够在更短的时间内生产皮肤替代物而不损害其内在组织特性，为目前可用的烧伤和伤口治疗提供了一个有前景的补充。
