Grundy M A, Coakley W T, Clarke D J
School of Pure and Applied Biology (Microbiology), University of Wales, College of Cardiff, UK.
J Clin Lab Immunol. 1989 Oct;30(2):93-6.
A haemagglutination test for Hepatitis B virus has been expedited by increasing cell-cell contact through concentrating antibody-coated erythrocytes in an ultrasonic standing wave field. The reactants used were taken from a commercially available Hepatitis B surface antigen screening kit, which is commonly employed in hospital laboratories and blood banks to screen human sera. When performed in microwells, as prescribed in the kit, positive and negative reactions were discernible after 30 min. However, with the ultrasonic technique these reactions can be distinguished within 5 min.
通过在超声驻波场中浓缩抗体包被的红细胞来增加细胞间接触，从而加快了乙型肝炎病毒血凝试验的速度。所用反应物取自市售的乙型肝炎表面抗原筛查试剂盒，该试剂盒常用于医院实验室和血库筛查人血清。按照试剂盒规定在微孔中进行检测时，30分钟后可辨别出阳性和阴性反应。然而，采用超声技术，这些反应在5分钟内即可区分。
