McDonald C P, Barbara J A, al-Izzi A, Ahya R, Vyas N, Contreras M
North London Blood Transfusion Centre, Colindale, UK.
Med Lab Sci. 1992 Mar;49(1):66-9.
Modification of a commercial haemagglutination assay for the detection of anti-HBs has reduced the test time from over one hour to approximately 25 min. increased sensitivity ten-fold without any prozoning, maintained specificity and reduced costs by 90%. The modification consists of diluting the reagent cells ten-fold; these are then added to dilutions of test serum in a V-well microplate. After incubation, plates are centrifuged and then inclined at 70 degrees. Positive and negative reactions can be clearly distinguished within approximately 10 minutes.
对用于检测抗-HBs的商业血凝试验进行的改进，已将检测时间从一个多小时缩短至约25分钟。灵敏度提高了十倍，不存在前带现象，保持了特异性，并将成本降低了90%。改进方法包括将试剂细胞稀释十倍；然后将其加入V型孔微量滴定板中的待测血清稀释液中。孵育后，对滴定板进行离心，然后倾斜70度。大约10分钟内即可清晰区分阳性和阴性反应。
