Derouin F, Rabian-Herzog C, Sulahian A
Laboratoire de Parasitologie Mycologie, Hôpital Saint-Louis, Paris, France.
J Clin Lab Immunol. 1989 Oct;30(2):97-102.
Specific cellular and humoral response to Toxoplasma were evaluated during a 2 yr follow up in a patient with acquired infection. Antibodies were titrated using conventional serological tests and analyzed by western blot technique. Analysis of the patterns obtained with IgA, IgG and IgM antibodies allowed a clear differentiation between acute and chronic stages of infection. Determination of lymphocyte subsets showed an important increase in the number of CD8 suppressive cells at the acute phase whereas CD4 cells remained within the normal range. Lymphocyte proliferation to Toxoplasma antigen was detectable one month after the onset of clinical symptoms and remained constantly positive. These results indicate that western blot analysis of antigens recognized by antibodies and determination of lymphocyte subsets may be helpful in the characterization of the infectious stage of acquired toxoplasmosis.
在对一名获得性感染患者进行的2年随访期间，评估了对弓形虫的特异性细胞和体液反应。使用传统血清学检测对抗体进行滴定，并通过蛋白质印迹技术进行分析。对IgA、IgG和IgM抗体获得的模式分析允许在感染的急性期和慢性期之间进行明确区分。淋巴细胞亚群的测定显示急性期CD8抑制细胞数量显著增加，而CD4细胞仍在正常范围内。临床症状出现一个月后可检测到淋巴细胞对弓形虫抗原的增殖，并且一直呈阳性。这些结果表明，抗体识别抗原的蛋白质印迹分析和淋巴细胞亚群的测定可能有助于获得性弓形虫病感染阶段的特征化。
