Crouch C F
Kodak Clinical Diagnostics Ltd, Pollards Wood Laboratories, Chalfont, St Giles, Bucks.
J Clin Pathol. 1995 Jul;48(7):652-7. doi: 10.1136/jcp.48.7.652.
To evaluate the clinical performance of enzyme immunoassays for IgG and IgM antibodies to Toxoplasma gondii based on enhanced chemiluminescence.
Classification of routine clinical samples from the originating laboratories was compared with that obtained using the chemiluminescence based assays. Resolution of discordant results was achieved by testing in alternative enzyme immunoassays (IgM) or by an independent laboratory using the dye test (IgG).
Compared with resolved data, the IgM assay was found to be highly specific (100%) with a cut off selected to give optimal performance with respect to both the early detection of specific IgM and the detection of persistent levels of specific IgM (sensitivity 98%). Compared with resolved data, the IgG assay was shown to have a sensitivity and a specificity of 99.4%.
The Amerlite Toxo IgM assay possesses high levels of sensitivity and specificity. Assay interference due to rheumatoid factor like substances is not a problem. The Amerlite Toxo IgG assay possesses good sensitivity and specificity, but is less sensitive for the detection of seroconversion than methods detecting both IgG and IgM.
评估基于增强化学发光的弓形虫IgG和IgM抗体酶免疫测定的临床性能。
将来自原始实验室的常规临床样本分类与使用基于化学发光的测定法获得的分类进行比较。通过在替代酶免疫测定法（IgM）中进行检测或由独立实验室使用染色试验（IgG）来解决不一致的结果。
与已解决的数据相比，发现IgM测定具有高度特异性（100%），所选临界值在特定IgM的早期检测和特定IgM持续水平的检测方面均具有最佳性能（敏感性98%）。与已解决的数据相比，IgG测定的敏感性和特异性分别为99.4%。
Amerlite弓形虫IgM测定具有高水平的敏感性和特异性。类风湿因子样物质引起的测定干扰不是问题。Amerlite弓形虫IgG测定具有良好的敏感性和特异性，但在检测血清转化方面不如同时检测IgG和IgM的方法敏感。
