柱前衍生化高效液相色谱法测定三七中人参皂苷Rg1和Re的含量

[Determination of Dencichine and Its Isomer in Panax notoginseng by Pre-column Derivatization HPLC].

作者信息

Yang Ling-juan, Jiao Cheng-jin, Gao Er-quan

出版信息

Zhong Yao Cai. 2015 Feb;38(2):311-4.

Abstract

OBJECTIVE

To establish a method to determine dencichine (β-ODAP) and its isomer (α-ODAP) in Panax notoginseng by pre-column derivatization HPLC with 1-fluoro-2,4-dinitrobenzene (FDNB).

METHODS

A Luna-C18 column (250 mm x 4.6 mm, 5 µm) was used, acetonitrile- (HAc-NaAc) as the mobile phase with the ratio of 17:83, the detection wavelength was 360 nm, the column temperature was 40 °C, and 20 µL of sample was injected for HPLC analysis.

RESULTS

In 0.30-50.50 µg/mL,β-ODAP had a good linear relationship with peak area, A2 = 140.50C1 + 72.30, r1 = 0.9995; In 0.10-16.15 µg/mL, α-ODAP has a good linear relationship with peak area, A2 = 106.60C2 + 56.00, r2 = 0.9992.

CONCLUSION

There are appreciable β-ODAP and α-ODAP in different samples of Panax notoginseng. The method is simple, rapid, accurate and can be used for the detection of dencichine and its isomer in other samples.

摘要

目的

建立一种采用1-氟-2,4-二硝基苯（FDNB）柱前衍生化高效液相色谱法测定三七中三七素（β-ODAP）及其异构体（α-ODAP）的方法。

方法

采用Luna-C18柱（250 mm×4.6 mm，5 µm），以乙腈-（HAc-NaAc）为流动相，比例为17:83，检测波长为360 nm，柱温为40℃，进样量为20 µL进行高效液相色谱分析。

结果

在0.30 - 50.50 µg/mL范围内，β-ODAP峰面积与浓度呈良好线性关系，A2 = 140.50C1 + 72.30，r1 = 0.9995；在0.10 - 16.15 µg/mL范围内，α-ODAP峰面积与浓度呈良好线性关系，A2 = 106.60C2 + 56.00，r2 = 0.9992。