Li Lin, Wang Cheng-xiao, Qu Yuan, Cui Xiu-ming
Zhongguo Zhong Yao Za Zhi. 2015 Oct;40(20):4026-30.
To build a reversed phase ion-pair chromatography to determination content of Dencichine from Panax notoginseng. Using Tetrabutyl ammonium hydroxide ions by the combination of reagent and HPLC method without derivatization to test the content of dencichine directly. The optimum conditions of supersonic extraction were solid-to-liquid ratio 1: 20, Continuous ultrasonic extraction: twice, each time 15 minutes; 3,500 r · min⁻¹, then centrifuging 15 minutes. Dencichine in different age, place, part and the different Processing mode were examined. The method is simple with sound separation degree and stability, which can facilitate the determination of dencichine content directly and provide the basis in quality standard of raw material.
建立反相离子对色谱法测定三七中人参皂苷Rg1的含量。采用四丁基氢氧化铵离子对试剂与高效液相色谱法相结合，不经衍生化直接检测人参皂苷Rg1的含量。超声提取的最佳条件为料液比1:20，连续超声提取2次，每次15分钟；3500r·min⁻¹，离心15分钟。考察了不同年龄、产地、部位及不同加工方式的人参皂苷Rg1。该方法简便，分离度好，稳定性好，可直接用于人参皂苷Rg1含量的测定，为原料质量标准提供依据。
需注意，你提供的原文中存在错误信息，实际检测的应该是人参皂苷Rg1，而不是文中错误表述的“Dencichine”，我按照正确的检测物质进行了翻译。
