Xie Guo-Xiang, Qiu Yun-Ping, Qiu Ming-Feng, Gao Xian-Fu, Liu Yu-Min, Jia Wei
School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, PR China.
J Pharm Biomed Anal. 2007 Feb 19;43(3):920-5. doi: 10.1016/j.jpba.2006.09.009. Epub 2006 Oct 6.
Dencichine (beta-N-oxalyl-l-alpha,beta-diaminopropionic acid) is a haemostatic agent present in well-known traditional Chinese medicinal herbs such as Panax notoginseng, as well as other Panax species. It is also a reported neurotoxic agent found in Lathyrus sativus (grass pea seed) and cycad seeds. A method was developed for quantitative determination of the non-protein amino acid, dencichine, in plant samples of P. notoginseng and the adventitious roots directly from the explants of P. notoginseng after derivatization with ethyl chloroformate (ECF) by gas chromatography-mass spectrometry (GC-MS). l-2-chlorophenylalanine was used as an internal standard. Calibration curves were linear (r(2)=0.9988, n=6) in the range of 10-800 microg/ml for dencichine. Limit of detection and quantification for dencichine were 0.5 microg/ml and 2 microg/ml, respectively. This rapid and specific method may be applied to the quantification of dencichine in complex medicinal plants and their products.
三七素（β-N-草酰-L-α，β-二氨基丙酸）是一种止血剂，存在于三七等著名的传统中药材以及其他人参属植物中。它也是一种在草豌豆（草豌豆种子）和苏铁种子中发现的具有神经毒性的物质。建立了一种用氯甲酸乙酯（ECF）衍生化后，通过气相色谱-质谱联用（GC-MS）定量测定三七植物样品及三七外植体直接诱导产生的不定根中三七素（一种非蛋白质氨基酸）的方法。L-2-氯苯丙氨酸用作内标。三七素在10-800μg/ml范围内校准曲线呈线性（r² = 0.9988，n = 6）。三七素的检测限和定量限分别为0.5μg/ml和2μg/ml。这种快速且特异的方法可用于复杂药用植物及其制品中三七素的定量分析。
