Wang Ting, Wei Zhi-feng, Dou Yan-nong, Xia Yu-feng, Dai Yue
Zhong Yao Cai. 2015 Feb;38(2):333-8.
To explore the changes of intestinal mucosal immunity in collagen-induced arthritis rats and the impact of madecassoside on these changes.
Collagen-induced arthritis was established in female Wistar rats. Treatment group was orally administrated madecassoside once daily for consecutive 21 days, while blank control and model groups were orally administered saline at the same volume. The concentrations of sIgA in small intestine content and IFN-γ in small intestinal tissue homogenate were determined by ELISA. The proportions of CD4+ T and CD8+ T in the epithelium and laminar propria of small intestine were detected by flow cytometry, and the ratios of CD4+/CD8+ were calculated. The relative expressions of CD80, CD86, IL-6, IL-12 and Foxp3 mRNA in the small intestine were determined by real-time PCR.
Compared with blank control rats, the concentrations of sIgA in small intestine content and IFN-γ in small intestinal tissue homogenate from model rats were increased, the ratios of CD4+/CD8+ in the epithelium and laminar propria of small intestine were higher and the relative expressions of CD80, CD86, IL-6 and IL-12 mRNA in the small intestine were increased. Madecassoside treatment decreased the concentrations of sIgA in small intestine content and IFN-γ in small intestinal tissue, downregulated the ratios of CD4+/CD8+ in the epithelium and laminar propria and decreased the relative expressions of CD80, CD86, IL-6 and IL-12 mRNA, while upregulated the relative expression of Foxp3 mRNA in the small intestine.
The intestinal mucosal immune response is enhanced in collagen-induced arthritis rats, the antigen presenting cells are activated abnormally and the immune tolerance is disturbed. Madecassoside treatment can downregulate the intestinal mucosal immune response and benefit for the induction and maintenance of intestinal immune tolerance.
探讨胶原诱导性关节炎大鼠肠道黏膜免疫的变化以及积雪草苷对这些变化的影响。
在雌性Wistar大鼠中建立胶原诱导性关节炎模型。治疗组连续21天每日口服一次积雪草苷，空白对照组和模型组则口服相同体积的生理盐水。采用ELISA法测定小肠内容物中sIgA的浓度以及小肠组织匀浆中IFN-γ的浓度。通过流式细胞术检测小肠上皮和固有层中CD4⁺T和CD8⁺T的比例，并计算CD4⁺/CD8⁺的比值。采用实时PCR法测定小肠中CD80、CD86、IL-6、IL-12和Foxp3 mRNA的相对表达量。
与空白对照组大鼠相比，模型组大鼠小肠内容物中sIgA的浓度以及小肠组织匀浆中IFN-γ的浓度升高，小肠上皮和固有层中CD4⁺/CD8⁺的比值升高，小肠中CD80、CD86、IL-6和IL-12 mRNA的相对表达量增加。积雪草苷治疗降低了小肠内容物中sIgA的浓度以及小肠组织中IFN-γ的浓度，下调了小肠上皮和固有层中CD4⁺/CD8⁺的比值，降低了CD80、CD86、IL-6和IL-12 mRNA的相对表达量，同时上调了小肠中Foxp3 mRNA的相对表达量。
胶原诱导性关节炎大鼠的肠道黏膜免疫反应增强，抗原呈递细胞被异常激活，免疫耐受受到干扰。积雪草苷治疗可下调肠道黏膜免疫反应，有利于诱导和维持肠道免疫耐受。
