Sharkey David J, Schjenken John E, Mottershead David G, Robertson Sarah A
Robinson Research Institute and School of Paediatrics and Reproductive Health, University of Adelaide, Adelaide, South Australia, Australia.
Robinson Research Institute and School of Paediatrics and Reproductive Health, University of Adelaide, Adelaide, South Australia, Australia.
Mol Cell Endocrinol. 2015 Dec 5;417:178-90. doi: 10.1016/j.mce.2015.09.022. Epub 2015 Sep 26.
Seminal fluid induces pro-inflammatory cytokines and elicits an inflammation-like response in the cervix. Here, Affymetrix microarray and qPCR was utilised to identify activin A (INHBA) and its inhibitor follistatin (FST) amongst the cytokines induced by seminal plasma in Ect1 ectocervical epithelial cells, and a similar response was confirmed in primary ectocervical epithelial cells. TGFB is abundant in seminal plasma and all three TGFB isoforms induced INHBA in Ect1 and primary cells, and neutralisation of TGFB in seminal plasma suppressed the INHBA response. Bacterial lipopolysaccharide in seminal plasma also elicited INHBA, but potently suppressed FST production. There was moderate reciprocal inhibition between FST and INHBA, and cross-attenuating effects were seen. These data identify TGFB and potentially LPS as factors mediating seminal plasma-induced INHBA synthesis in cervical cells. INHBA and FST induced by seminal fluid in cervical tissues may thus contribute to regulation of the post-coital response in women.
精液可诱导促炎细胞因子，并在宫颈引发类似炎症的反应。在此，利用Affymetrix微阵列和定量聚合酶链反应（qPCR）在Ect1宫颈外上皮细胞中由精浆诱导产生的细胞因子中鉴定激活素A（抑制素βA亚基，INHBA）及其抑制剂卵泡抑素（FST），并在原代宫颈外上皮细胞中证实了类似反应。转化生长因子β（TGFB）在精浆中含量丰富，所有三种TGFB亚型均在Ect1细胞和原代细胞中诱导INHBA产生，精浆中TGFB的中和作用抑制了INHBA反应。精浆中的细菌脂多糖也引发INHBA产生，但有力地抑制了FST的产生。FST和INHBA之间存在适度的相互抑制，并观察到交叉衰减效应。这些数据确定TGFB以及潜在的脂多糖是介导精浆诱导宫颈细胞中INHBA合成的因素。因此，精液在宫颈组织中诱导产生的INHBA和FST可能有助于调节女性性交后的反应。
