在250兆赫频率下对二硫化二硝基化合物进行成像以监测硫醇氧化还原状态。
Imaging disulfide dinitroxides at 250 MHz to monitor thiol redox status.
作者信息
Elajaili Hanan, Biller Joshua R, Rosen Gerald M, Kao Joseph P Y, Tseytlin Mark, Buchanan Laura A, Rinard George A, Quine Richard W, McPeak Joseph, Shi Yilin, Eaton Sandra S, Eaton Gareth R
机构信息
Department of Chemistry and Biochemistry, University of Denver, Denver, CO 80208, United States.
Department of Pharmaceutical Sciences and Center for Biomedical Engineering and Technology, University of Maryland School of Medicine, Baltimore, MD 21201, United States.
出版信息
J Magn Reson. 2015 Nov;260:77-82. doi: 10.1016/j.jmr.2015.08.027. Epub 2015 Sep 12.
Abstract
Measurement of thiol-disulfide redox status is crucial for characterization of tumor physiology. The electron paramagnetic resonance (EPR) spectra of disulfide-linked dinitroxides are readily distinguished from those of the corresponding monoradicals that are formed by cleavage of the disulfide linkage by free thiols. EPR spectra can thus be used to monitor the rate of cleavage and the thiol redox status. EPR spectra of (1)H,(14)N- and (2)H,(15)N-disulfide dinitroxides and the corresponding monoradicals resulting from cleavage by glutathione have been characterized at 250 MHz, 1.04 GHz, and 9 GHz and imaged by rapid-scan EPR at 250 MHz.
摘要
硫醇-二硫化物氧化还原状态的测量对于肿瘤生理学特征的表征至关重要。二硫键连接的二氮氧化物的电子顺磁共振（EPR）光谱很容易与相应的单自由基的光谱区分开来，这些单自由基是由游离硫醇裂解二硫键形成的。因此，EPR光谱可用于监测裂解速率和硫醇氧化还原状态。（1）H，（14）N-和（2）H，（15）N-二硫二氮氧化物以及由谷胱甘肽裂解产生的相应单自由基的EPR光谱已在250 MHz、1.04 GHz和9 GHz下进行了表征，并在250 MHz下通过快速扫描EPR进行了成像。
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