Kido Hueliton W, Brassolatti Patricia, Tim Carla R, Gabbai-Armelin Paulo R, Magri Angela M P, Fernandes Kelly R, Bossini Paulo S, Parizotto Nivaldo A, Crovace Murilo C, Malavazi Iran, da Cunha Anderson F, Plepis Ana M G, Anibal Fernanda F, Rennó Ana C M
Department of Biosciences, Federal University of São Paulo (UNIFESP), Santos, Sao Paulo, Brazil.
Department of Physiotherapy, Post-Graduate Program of Biotechnology, Federal University of São Carlos (UFSCar), São Carlos, Sao Paulo, Brazil.
J Biomed Mater Res B Appl Biomater. 2017 Jan;105(1):63-71. doi: 10.1002/jbm.b.33536. Epub 2015 Sep 28.
This study evaluated the effects of the Biosilicate and poly (D,L-lactic-co-glycolic) acid composites on bone repair in a tibial bone defect model in rats by means of using histological evaluation (histopathological and morphometric analysis) and gene expression analysis. Eighty male Wistar rats (12 weeks old, weighing ±300 g) were randomly divided into two groups: Biosilicate group (BG) and Biosilicate /PLGA group (BG/PLGA). Each group was euthanized at 3, 7, 14, and 21 days after surgery (n = 10 animals per time point). The main findings showed that the incorporation of PLGA into BG had a significant effect on the morphological structure of the material, accelerating mass loss, decreasing the pH and increasing the calcium release. Furthermore, histologic analysis revealed that the BG/PLGA showed increased material degradation, accompanied by higher bone formation compared to BG, after 21 days of implantation. In addition, qRT-PCR analysis showed that BG/PLGA induced an upregulation of the osteogenic genes related to BMP4, Runx2, ALP, and OC. These results show that the present BG/PLGA composite may be used as a bone graft for inducing bone repair. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 63-71, 2017.
本研究通过组织学评估（组织病理学和形态计量分析）以及基因表达分析，评估了生物硅酸盐与聚（D，L-乳酸-共-乙醇酸）酸复合材料对大鼠胫骨骨缺损模型中骨修复的影响。80只雄性Wistar大鼠（12周龄，体重约300g）被随机分为两组：生物硅酸盐组（BG）和生物硅酸盐/聚乳酸-羟基乙酸共聚物组（BG/PLGA）。每组在术后3、7、14和21天实施安乐死（每个时间点n = 10只动物）。主要研究结果表明，将聚乳酸-羟基乙酸共聚物加入生物硅酸盐中对材料的形态结构有显著影响，加速了质量损失，降低了pH值并增加了钙释放。此外，组织学分析显示，植入21天后，BG/PLGA的材料降解增加，与BG相比，骨形成更高。此外，定量逆转录-聚合酶链反应分析表明，BG/PLGA诱导了与骨形态发生蛋白4、 runt相关转录因子2、碱性磷酸酶和骨钙素相关的成骨基因上调。这些结果表明，目前的BG/PLGA复合材料可作为诱导骨修复的骨移植材料。© 2015威利期刊公司。《生物医学材料研究杂志》B部分：应用生物材料，105B：63 - 71，2017年。
