Kido Hueliton Wilian, Tim Carla Roberta, Bossini Paulo Sérgio, Parizotto Nivaldo Antônio, de Castro Cynthia Aparecida, Crovace Murilo Camuri, Rodrigues Ana Candida Martins, Zanotto Edgar Dutra, Peitl Filho Oscar, de Freitas Anibal Fernanda, Rennó Ana Claudia Muniz
Department of Physiotherapy, Post-Graduate Program of Biotechnology, Federal University of São Carlos (UFSCar), São Carlos, SP, Brazil,
J Mater Sci Mater Med. 2015 Feb;26(2):74. doi: 10.1007/s10856-015-5411-9. Epub 2015 Jan 29.
The aim of this study was to evaluate the effects of highly porous Biosilicate(®) scaffolds on bone healing in a tibial bone defect model in rats by means of histological evaluation (histopathological and immunohistochemistry analysis) of the bone callus and the systemic inflammatory response (immunoenzymatic assay). Eighty Wistar rats (12 weeks-old, weighing±300 g) were randomly divided into 2 groups (n=10 per experimental group, per time point): control group and Biosilicate® group (BG). Each group was euthanized 3, 7, 14 and 21 days post-surgery. Histological findings revealed a similar inflammatory response in both experimental groups, 3 and 7 days post-surgery. During the experimental periods (3-21 days post-surgery), it was observed that the biomaterial degradation, mainly in the periphery region, provided the development of the newly formed bone into the scaffolds. Immunohistochemistry analysis demonstrated that the Biosilicate® scaffolds stimulated cyclooxygenase-2, vascular endothelial growth factor and runt-related transcription factor 2 expression. Furthermore, in the immunoenzymatic assay, BG presented no difference in the level of tumor necrosis factor alpha in all experimental periods. Still, BG showed a higher level of interleukin 4 after 14 days post-implantation and a lower level of interleukin 10 in 21 days post-surgery. Our results demonstrated that Biosilicate® scaffolds can contribute for bone formation through a suitable architecture and by stimulating the synthesis of markers related to the bone repair.
本研究旨在通过对骨痂进行组织学评估（组织病理学和免疫组织化学分析）以及对全身炎症反应进行免疫酶测定，来评估高度多孔的生物硅酸盐支架对大鼠胫骨骨缺损模型中骨愈合的影响。将80只Wistar大鼠（12周龄，体重±300克）随机分为2组（每个实验组、每个时间点n = 10）：对照组和生物硅酸盐组（BG）。每组在术后3、7、14和21天实施安乐死。组织学结果显示，术后3天和7天，两个实验组的炎症反应相似。在实验期间（术后3 - 21天），观察到生物材料的降解主要发生在外围区域，这促使新形成的骨向支架内生长。免疫组织化学分析表明，生物硅酸盐支架刺激了环氧化酶 - 2、血管内皮生长因子和与 runt 相关的转录因子2的表达。此外，在免疫酶测定中，BG在所有实验期间肿瘤坏死因子α水平均无差异。不过，BG在植入后14天白细胞介素4水平较高，术后21天白细胞介素10水平较低。我们的结果表明，生物硅酸盐支架可以通过合适的结构并刺激与骨修复相关标志物的合成来促进骨形成。
